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1 <br /> I <br /> i A. Keith Kaufman, M.S. <br /> Consultant in Environmental Biotechnology <br /> Serving Industry,Government,Academia and the Legal Profession <br /> ' September 9, 1999 <br /> TO: Mr. Bill Little (Advanced GeoEnviron mental, Inc,) <br /> FROM: A. Keith Kaufman (�% <br /> Subject: Results of Physicochemical and Microbiological Analyses of Three (3) Soil <br /> Samples and One (1) Water Sample (Olympian Oil) <br /> ' Listed below are the results from the referenced analyses performed on three (3) soil samples <br /> (MW2-10, MW2-20, & MW2-30 ) and one (1) water sample (MW-1 ) collected on 8/25199 <br /> ' (water sample) and 8/19/99 (soil samples) and received on 8/27/99. After appropriate <br /> dilution, samples were plated onto TSA and MSA supplemented with 100 and 500 ppm of <br /> diesel fuel (for soil samples), and 500 ppm diesel + 50 ppm gasoline (for water sample) as <br /> sole carbon substrates. Media were prepared at ambient pH. Cultures were incubated under <br /> aerobic conditions at room temperature for six (6) days prior to enumeration. Physicochemical <br /> analyses were performed in accordance with previously described protocol. <br /> 1 RESULTS <br /> MParameie� 111J]bL-1_ MW2-10 MW2-20 MW2-30 <br /> t pH 7.4 9.4 9.7 9.6 <br /> Nitrate-N (ppm) 1 .4 15.0 6.0 6.0 <br /> Nitrate (ppm) 6.2 66.0 26.4 26.4 <br /> ' Ammonia-N (ppm) 0.8 8.0 3.0 ND <br /> Ammonium (ppm) 1 .0 10.4 3.9 ND <br /> Ortho-P (ppm) 1 .1 1 1 .7 13.8 14.1 <br /> ' Potassium (ppm) 6.0 114 186 117 <br /> ' % Moisture --- 17.8 17.5 18.4 <br /> Gen. Mier. Enum. <br /> ' (CFU X 105/ml/gr) 22.0 1 .9 0.10 0.34 <br /> Sel, Mier. Enum. <br /> ' (CFU) 105/ml/gr) 14.2. 1 .9.1 0.06- 0.02 <br /> Biodegraders - .64.5% 1066/, 60%, 176/,, ; <br /> ND; Not detected at limit of 0.1 ppm/10-2 dilution <br /> ,CFl1; Colnnv-forming LlnitS-,,(�ij,L�r.PIIG4 <br /> United States Office Asia-Pacific Office <br /> ' 6245 Bristol Parkway#249 2nd Floor,#162 Tung-An Street,Ta-An District <br /> Culver City,CA 90230 Taipei,Taiwan R.O.C. <br /> (310)822-9385 (310)577-9364[FAX) 886-2-378-3751 886-2-378-3752{FAX] <br />