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FGL ENVIRONMENTAL 853 Corporation St. - P.O. Box 272 Santa Paula , California 93060 7: ,p 1 96 HOUR STATIC BIOASSAY - TITLE 22 DEFINITIVE 3-2- Lab No. 13183-2 Client: Energy Factors 2526 W. Washington St. Stockton, CA 95203 Sample Description: 1-B Bottom Ash Composite Sampled by: Energy Factors Date Sampled: 3/3/89 Date Received: 3/7/89 Date Reported: 3/22/89 REPORT OF ANALYSIS Test Initial 24 hrs. 48 hrs. 72 hrs. 96 hrs. FINAL INITIAL Con'c. pH DO Temp. No. pH DO Temp. No. pH DO Temp. No. pH DO Temp. No. pH DO Temp. % CaCO3, m /l % mg/l °C Dead * mg/l °C Dead mg/l °C Dead mg/l °C Dead mg/l °C Mortality <a- Hard- linit ness Control 8.1 7.7 20 0 7.7 9.8 17 0 7.8 9.2 17 0 7.9 9.2 17 0 8.0 9.3 17 0 36 46 18 7.8 7.3 20 0 7.6 8.1 17 0 7.5 6.4 17 0 7.7 7.9 17 0 7.6 5.1 17 0 -- 32 7.4 7.3 20 I 0 8.8 8.8 17 0 7.7 8.5 17 0 7.8 8.7 17 0 7.7 8.7 17 0 -- -- 56 8.0 7.4 20 0 9.9 9. 1 17 1 7.8 8.8 17 0 7.8 9.0 17 I 0 7.8 8.9 17 I 5 I -- -- 75 8.2 7.7 20 20 10.5 9.6 17 -- 9.6 9.4 17 0 8.9 9.4 17 0 7.9 9.0 17 I 100 -- 100 8.3 7.7 20 20 10.9 9.7 17 -- 10.0 9.5 17 0 9.2 9.6 17 0 8.9 8.5 17 I 100 16 176 Species: Fathead Minnow Ave. Length: .8 cm Max. Length: 3.0 cm Min. engt : 2.6 cm Avg. Weight: 2.9_2 Max. Weight: 1. 1_2 Min. Weight. 0.7 g Number of test animals per concentration: 20 Volume of test solution: 10 Liters Depth: 12.5 cm Type Aeration: Continuous Acclimatization 10 days at 18 °C. Percent dead in acclimatization tank 0 Water Source Deionized + Minerals LC50 Greater than 500 mg/L % Survival undiluted waste 0 Dilution Water Source Acclimation Water * pHs were adjusted but returned to original pH. Can' t readjust once bioassay is started. Sample appeared "soapy" . Ana 1 ys t ,4C/YL9_1 A waste sample is designated as hazardous (Section 4, Chapter 30, Article 11 of Title 22 of the California Administrative Code) if the LC50 is less than 500 mg/liter.