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BioJet <br /> Innovative Remediation Technologies <br />� f <br /> April 17, 2002 <br /> A.E.S. Regulatory Services <br /> Attn Mr Greg Sengelmann <br /> 2013 Wells Branch Parkway, Suite 206 <br /> Austin, Texas 78728 <br /> Phone 512-990-7467 Fax 512-990-7399 <br /> Subject Final Report for the bench scale investigations of site-specific samples obtained <br /> from the former Canteen Corp, in Stockton Ca <br /> Dear Mr Sengelmann <br /> Bio7et, Innovative Remediation Technologies is pleased to provide A E S Regulatory <br /> Services (AES) this final report describing the procedures, cumulative results, and <br /> recommendations for implementation of remediation at the subject site The data for this <br /> report was generated from two separate studies conducted on soil samples from Stockton, <br /> 1 CA <br /> The primary purpose for undertaking these studies was to determine what, if any, <br /> physical parameters could be adjusted to allow for increased microbial populations, <br /> specifically the contaminant degraders, which serve to expedite bioremediation time <br />' frames The studies that were conducted were as follows <br /> 1 )Effects of Nutrient Augmentation on Microbial Growth <br /> This study is conducted to determine if nutrient deficiencies may be a contributing <br /> factor limiting bioremediation for site-specific samples and whether and to what <br />' degree the addition of nutrients would enhance microbial populations <br /> 2 } Erects of Oxygen Augmentation <br />' Oxygen deficiency in in situ environments is a well-documented limiting factor to <br /> aerobic biodegradation processes The purpose of evaluating the effects of <br />' oxygen supplementation is to determine if increased levels of oxygen can promote <br /> microbial growth and biodegradation within the contaminated matrix Site- <br /> specific samples are enriched with varying concentrations of oxygen Following <br />' an adaptation period, microbial enumerations are performed on the oxygen- <br /> eririched samples and the differences in microbial o <br /> iagrowth patterns, if any, are <br /> observed A comparison of baseline microbial enumerations with the results from <br /> these evaluations can be used to determine if oxygen is acting as a growth- <br /> limiting factor <br />' 14025 willow Creek Road Ione, CA 95640 • 209-245-6044 • Fax 209-245-3765 <br />