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&LoJet <br /> Innovative Remediation Technologies <br /> Mr Greg Sengelmann Protect Name Fomtes Canteen Corp Sampled 02/25/02 <br /> A.E S Regulatory Services Client Project 1D Received 22/27/02 <br /> Plated 42/27/02 <br /> 2013 Wells Branch Parkway,Suite 206 Sampled By 02128/02Austin,Texas 78728 Analysis Run Microbial&Physicochemtcai Enumerations Analyzed(Physio) 03/25/02 <br /> Phone 512-990-7467 Fax 512-990-7399 Laboratory lndentification BJ-AES-FCC-1-4 Enumerated 03/07/02 <br /> P O # Plating Concentration 1000 ppm gasoline Reported <br /> Site Location Stockton Ca BioJet Pro ect Manager Ken Farrar 209-245-6044 Fx 209-245-3765 <br /> Listed below are the results of microbial and physicochemical analyses,performed on four(4)soil samples collected on February 27 2002 from the Former <br /> Canteen Corp site and rece-ved by BioJet on February 27,2002 <br /> Samples were analyzed for pH and concentrations of nitrogen(nitrate, ammonia),phosphorus(as ortho-phosphate)and potassium utilizing a Hach DR2000 <br /> spectrophotomenter and pH meter in accordance with the manufactures instructions General(heterotrophic,nonspecific)and selective(Gasoline specific) <br /> enumerations were performed,respectively,on Plate Count Agar(nutritionally complex)and 50%Bushnell-Haas minimal salts media supplemented wrath 1000 <br /> ppm gasoline,as the sole carbon source Using standard microbiological plate count techniques,serial dilutions of each water sample were inoculated onto each <br /> media type and incubated aerobically,for six(6)days at 30 degrees Celsius prior to evaluation A single dilution for each sample were perfom led In tnpllcate <br /> with an average of all three being reported for each sample <br /> Laboratory Results" <br /> Sample GEN SEL DEG <br /> Sample ID 4 DescnpLon pH NO,N NO3 NH,N NH, PO, K 1 00E+05 1 00E+05 % <br /> VE-1 @ 25' 71 61 2684 ND ND 21 140 8 001 013% <br /> 2 VE-1 @ 40' 71 73 3212 ND ND 27 120 12 003 025% <br /> 3 SBI 4 @ 25' 75 10 44 3 387 33 95 17 1 1 647% <br /> 4 SB14 @40' 76 9 396 5 645 37 85 14 13 929% <br /> Signature <br /> Ken F44,Project Manager <br /> MC= mms4 re 0onoant NOrN= Nitrite-Nitrogen(ppm) PO,= Ortho-Phosphate(Ppm) <br /> pH=log Hydrog <br /> en ion Concentration NO.= N etrite(ppm) x=Potassnun(pprrt) GEN= Heterotrophic Organisms(CFU x 10� <br /> Nitrate-Nitrogen(Ppm) NH,N=Ammonia Nitrogen(ppm) Detection Limits for SEL=Serceroechve Degrader organismsDegrader Org 1rxmsrns <br /> N603= PerceiNage of Selective Degrader 9a <br /> N4,= Nrtrate Ion(ppm} N H.=Ammonium Ion(ppm) rnorgarvcs=001-0 1 ppm 96= <br />