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SEQUOIA ANALYTICAL DUH p 8 <br /> 1900 Bates Avenue • Suite LM • Concord California 94520 <br /> 45101 686 9600 • F-,x 1510) 686 9689 <br /> Olton Geoscience Client Project ID Mobil #04-NP4/ 30-0140-02 Sampled May 27 1992 <br /> 5870 Stoneridge Drive, Suite 6 Sample Descript Water, E-1 Received May 27, 1992 <br /> Pleasanton, CA 94588 Analysis Method See below <br /> Attention Cyril Yong Lab Number 2051243 Reported Jun 1, 1992 <br /> STATIC ACUTE EFFLUENT BIOASSAY <br /> Static X Species Oncorhynchus mykiss Organisms/Tank 10 <br /> Cont FlowCommon Name Rainbow Trout Replicates 1 <br /> Mean length 25 mm Organisms/Cone 10 <br /> Mean weight 0 20 g Tank Depth 13 cm <br /> Screening" Supplier Stickfebacks Unlimited Tank Volume 10 L <br /> Definitive X Acclimation Temp 12 degrees C <br /> Alkalinity mg/L Hardness mg/L <br /> Control 30 46 <br /> 10% 50 150 <br /> Dilution Water Synthetic Hardwater 18% 50 170 <br /> 32% 50 220 <br /> 56% 50 350 <br /> 100% 50 1600 <br /> Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> . DATE 5/28/92 5/29/92 1 5/30/92 5/31/92 6/1/92 <br /> D PH D PH # M D PH # M D PH # M D pH # M Total <br /> mg/L Temp Units mg/L Tem Units Dead mg/L Tem Units Dead mg/L Tem Units Dead mg/L Temp Units DeaC Dead <br /> Control 99 12 76 100 12 71 0 100 12 74 0 99 12 72 0 97 13 72 0 0 <br /> 10% 97 12 74 98 12 70 0 99 12 74 0 100 12 72 0 98 13 72 0 0 <br /> 18% 98 12 70 99 12 69 0 100 12 74 0 98 12 72 1 96 13 72 1 1 <br /> 32% 95 12 J64 <br /> 97 12 68 Q 100 12 73 0 99 12 72 1 97 13 72 1 1 <br /> 5696 90 12 97 12 66 0 100 12 72 0 98 72 71 1 96 13 71 1 1 <br /> 100% 90 12 98 12 65 0 1Q0 12 72 0 101 12 72 1 98 13 72 1 1 <br /> LC-50: > 100% LC-50 Calculation Method Moving average angle <br /> Remarks <br /> Analyst D George Method Reference Methods for Measunng the Acute Toxicity of Effluents to Freshwater <br /> and Manne Organisms, 3rd Ed , EPA 600/4-85/013 <br /> SEQUOIA ANALYTICAL <br /> Scott A Chieffo <br /> 4 <br /> Project Manager <br /> 20512-= ALT <4 7 <br />