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24 (1) Sample collection; (2) Sample preservation and shipment; (3) Analytical procedures; and (4) Chain of custody control . (b) The owner or operator shall determine the concentration or value of the following parameters in groundwater samples in accord- ance with Item 2(c) and (d) : (1) Parameters characterizing the suitability of the ground water as a drinking water supply as specified by EPA in Appendix III , Part 265, Title 40, Code of Federal Regulations. (2) Parameters establishing ground-water quality: (i) Chloride (ii) Iron (iii) Manganese (iv) Phenols (v) Sodium (vi) Sulfate (3) Parameters used as indicators of ground-water contamination: (I) pH (ii) Specific Conductance (iii) Total Organic Carbon (iv) Total Organic Halogen (c) (1) For all monitoring wells, the owner. or operator shall establish ini"tial. 4ackground concentrations or values of :all parameters specified, in Item 2C6.) . This shall be done quarterly for one year. (2) For each of the indicator parameters specified in Item 2 (b) (3) , at least four replicate measurements shall be obtained for each sample and the initial background arithmetic mean and variance shall be determined by pooling the replicate measurements for the respective parameter concentrations or values in samples obtained from upgradient wells during the first year. (d) After the first year, all monitoring wells shall be sampled and the samples analyzed with the following frequencies: (1) Samples collected to establish ground-water quality shall be obtained and analyzed for the parameters specified in Item 2(b) (2) at least annually. (2) Samples collected to indicate groundwater contamination shall be obtained and analyzed for the parameters specified in Item 2(b) (3) at least semi-annually.