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Biotechnical Laboratory and Consulting Sennoes <br /> Surnmaa ofAng&ses (Lab ID BL-RE-AT 1-3) <br /> BloLogic is pleased to offer Ramage Environmental the following observations regarding the <br /> results of microbial and physicochemical analyses performed on water samples collected from the <br /> AG Tracy site Please keep In mind that these findings were predicated only on data generated <br /> from BnoLogic To effectively provide a complete interpretation of site-specific microbial <br /> dynamics and present appropriate recommendations, we would require additional information <br /> regarding current site operations, particularly those pertaining to nutrient supplementation. <br /> For a site undergoing active bioremediation, it appeared that both general (heterotrophic, <br /> nonspecific) and selective (contaminant-specific) microbial population numbers were relatively <br /> low These low recoveries could be attributed to any number of environmental factors, for <br /> example, limited TPH concentrations, nutrient deficiencies, or conversely, nutrient overloading <br /> Another factor which often contributes to low population counts is the presence of"swarming" <br /> bacteria that spread over the surface of a culture effectively preventing the growth of other <br /> organisms through competition for available space Varying degrees of swarmers were observed <br /> on the majority of the plates counted which may suggest that the actual population numbers may <br /> be higher than those reported <br /> An important parameter in presenting a microbiological profile of a site is determining the relative <br /> percentage of selective degraders to the overall microbial population For the samples evaluated, <br /> the relative percentage of selective microorganisms to the total number of heterotrophs ranged <br /> from 21 05 to 41 18% Generally, as a rule of thumb, a percentage in the range of 20% or higher <br /> typically Is considered acceptable for achieving a degree of contaminant biodegradation <br /> Although the percentage of degraders would be considered good, the overall number of microbes <br /> is low <br /> he site 4 84 - 21.12 rn <br /> The Physicochemical data indicated that current nitrate levels at t ( ppm), <br /> appeared to be low at this time All other parameters analyzed for physicochem istry were within <br /> acceptable ranges <br /> BioLogic thanks Ramage Environmental for the opportunity to provide you this report, should <br /> you have any questions please contact your site project manager, Ken Farrar, at 209-245-4596 <br /> I <br />