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1. INTRODUCTION The California Department of Substances Control has adopted regulations(R-45-78) which define criteria for the identification of hazardous wastes. These criteria are codified in Chapter 11,Article 3 of Title 22 of the California Code of Regulations. Toxicity to aquatic life, specifically fish, is one of the criteria used to gauge the hazardous potential of a waste. An acute 96-hour bioassay is used to determine the LC50 as defined in Section 66261.24(a)(6)of these regulations. This 96-hour LC50 value serves as the numerical indicator of the toxicity of a waste to aquatic life. The sample is deemed hazardous if the LC50 is less than 500 mg/L. This report describes the procedures used and the results obtained for the hazardous waste aquatic toxicity screening test(s)performed by Block Environmental Services (BES) for Alpha Analytical. BES is an Environmental Laboratory Accreditation Program certified laboratory(#1812). 2. MATERIALS AND METHODS 2.1 TEST ORGANISMS • Rainbow Trout, Oncorhynchus mykiss,obtained from a commercial supplier. 2.2 TEST PROCEDURES A detailed procedure for this test is outlined in DPHS-ELAP approved standard operating procedures (SOPs),which are on file at the BES laboratory. These SOPs are based upon the following references: • California's Title 22 Code,Section#66261.24(a)(6);Static Acute Bioassay Procedures for Hazardous Waste Samples, Polisini and Miller, 1988,California Department of Fish and Game • Guidelines for Performing Static Acute Toxicity Fish Bioassays in Municipal and Industrial Wastewaters,Kopperdahl, 1976, California Department of Fish and Game • Standard Methods for the Examination of Water and Wastewater, I SP Edition,American Public Health Association, 1995. 2.3 DATA ANALYSIS All toxicity testing results will be analyzed using an appropriate statistical method to determine a LC50 and the corresponding 95%confidence limits. BES #28069 Page 2 of 3