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I . r <br />' 18 July 2002 <br /> AGE-NC Project No 96-0254 <br /> Page 14 of 17 <br /> degrade hydrocarbons and spread laterally by migrating and reproducing, creating an advancing <br />' remediation front <br /> 6 2 1 Nutrient Requirements And Toxicity <br />' The bacteria are not tolerant to a wide range of ground water conditions For instance, pH should <br /> range from 6 to 8 5 In addition, the possible presence of high concentrations of pesticides or <br /> fumlcldes at the site may have a biotoxic effect on some bacteria <br />' The microbes require certain essential nutrients that are commonly available In ground water If <br /> supplemental nutrients are required, such as nitrogen and phosphorus as ammonium phosphate in <br />' liquid phase,the quantity is usually very low However, a Waste Discharge Permit is required by the <br /> RWQCB for the infection of nutrients into ground water <br /> Typically,the microbes utilized in bioremediation are aerobic and usually require additional oxygen <br /> to supplement dissolved oxygen concentrations in ground water Generally, several air sparging or <br /> in-well aeration sources are sufficient to provide the necessary oxygen In some cases, addition of <br /> hydrogen peroxide is beneficial <br />' 6 2 2 Monitoring Activities <br /> After inoculation,the inoculation wells and ground water monitoring wells should be monitored for <br /> several months by periodic field measurements of organic vapor, carbon dioxide, and dissolved <br /> oxygen concentration Ground water samples should be collected and analyzed for petroleum <br /> hydrocarbons, bacterial populations and nutrient availability If monitoring indicates areas of slow <br /> Iremediation, additional inoculum and/or nutrients may be required <br />' 6 2 3 Feasibility Studies <br />' A limited feasibility study for in-situ bioremediation of the ground water at the site was performed <br /> in January 2002 Microbiological analysis of an impacted water sample indicated that populations <br /> of indigenous hydrocarbon-degrading bacteria constitute 29%ofthe total microbiological population <br />' at the site, and that some degree of contaminant biodeegradation is likely to be occurring The <br /> contaminant biodegradation is moderate to high due to high levels of nitrogen and phosphates The <br /> rate of contaminant biodegradation may be stimulated by the addition of nutrients into the ground <br /> Iwater Results of the microbiological and physicochemical analysis are Included in Appendix D <br /> Advanced GeoEnvironmental,Inc <br />