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Pacific EcoRisk Environmental Consulting and Testing <br /> The test was initiated with the random allocation of 10 juvenile fathead minnows into each <br /> replicate container. These containers were placed in a temperature-controlled room at 20°C, <br /> under fluorescent lighting on a 16L:8D photoperiod. <br /> Each day, each replicate container was examined, and the number of live fish in each was <br /> recorded. Routine water quality characteristics (temperature, pH, and D.O.) of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96 (±2) hours, the test was terminated and the number of live fish in each replicate <br /> container was determined. The fish in a randomly selected Lab Control treatment replicate were <br /> then euthanized and measured for total length and weighed. The resulting survival data for the <br /> waste treatments were evaluated to determine the LC50 using CETIST"(TidePool Scientific <br /> Software, McKinleyville, CA). <br /> 3. RESULTS <br /> 3.1 Acute Effects of the Waste Sample on Fathead Minnows <br /> The results of this test are summarized in Table 1. The LC5o of this sample was>750 mg/L, <br /> which is greater than the hazardous waste classification threshold of 500 mg/L, as defined by CA <br /> Title 22 CCR §66261.24. Accordingly, the sample should not be considered a hazardous waste <br /> with respect to acute aquatic toxicity. The test data and summary of statistics for this test are <br /> presented in Appendix B. <br /> Table 1. Results of the hazardous waste acute toxicity evaluation of the waste sample. <br /> Waste Sample Treatment Level Mean % Survival <br /> Lab Control 100 <br /> 250 mg/L 100 <br /> 500 mg/L 100 <br /> 750 mg/L 100 <br /> Summary of Key Statistic <br /> LC50 = 1 >750 mg/La <br /> a-Due to the absence of significant mortalities,the LC50 cannot be calculated,but can be determined by <br /> inspection to be>750 mg/L. <br /> Page 2 �))� <br /> 5/13 (( <br />