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0 <br /> I. INTRODUCTION <br /> The Calibrnia I) ent of-Substances Control has adapted regulations(R:•45-78)which <br /> deftne criteria for the identification ofhwWous wastes. Those criteria are codified..in Chapter <br /> 11,Article 3 of Title 22 of the California Cade of Regulati.ous. Twdeityto aquatic life, <br /> specifically Pah,is one of the criteria teria gauge the hazardous potential of a waste. An acute <br /> 96-hour bioassayls used to determine the.LC50 as deed in Se don 66261,24<a)(6)of these <br /> ations. This 96 LC50 value serves as the numerical indicator of,the tcaxeity of waste <br /> to aquatic.life. The sample is downed.h us iftheLdSO.isless than 500 mg/L. <br /> This report describes the.procedures used and the xesWts.obtained fortlae hazardous waste <br /> aquatic toxicity screening tests)pe rformied by B10ok Environmental:Services(EES)for <br /> Environ. <br /> BES is an Environmental Laboratory Aoc reditation Prograra oatlfibd laboratory(41012). <br /> I. KATEIUALS AND MZMODSKATE. <br /> 2.1 TEST ORGANISMS <br /> • Rainbow Trout,Oncorhynchus nxykiss,obtainecC.froln a commemial supplier. <br /> 2.2 TEST PKOCEDVM <br /> A detailed.procedure for this test is outlined in:laboratory standard opemlin procedures(SQPs) <br /> kWattheBESlaboraory. These SOPS are based uponthe fbllowin"itfamneer <br /> « Califomia'-s Title 22 Code,:3ecdon#66261:24(x, 6) Static: to Storiss <br /> Proceduresfar Hazardous haste Sample,Polisini a'n4 Miller, 1488,Cal forma <br /> • <br /> Dpparftent of Fish:and <br /> • Guldefines for Performing S14ffc Acute nxid*F`kh BloasBays hr Man cipal and <br /> Industrta7 Wastewaters,Kopperdahl, 1976;:California D artment of Fish and tame <br /> • Stoulard Methodsfir•0�e&=fnaHon of Water and Wastewater, 19th2dition, <br /> Amed PubHe H alth Association, 1995. <br /> 2.3 DATA ANALYSIS <br /> All toxicity testing results will be atWyzed using an Vptopriate statistical method to deter tie a <br /> LC50 and the corresponding 95 eonfiddace limits. <br /> BES#21385 Page 2 of 3 <br />