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• e <br /> 1. INTRODUCTION <br /> The California Depwtm=t of Substances Control has adopted re9d ation}s(R-45-78)which <br /> demo criteria for the identification of.bazartlous wastes, a criteria are codified in.Chapter <br /> 11,Article 3 ofTitle 22 of the California Code ofRegiations. Toxicity to aquatic liff'. <br /> agecif catly fish, is one of the criteria used to Saauge the hazardous potential of a waste. An acute <br /> 96-hour bioassay is,used to determine the LC50 as de ed ut Section 66261.24(aX.6)of these <br /> regulations. This 96-hour LCSU value smTes as the.numerical indicator of the toxicity of a <br /> to aquatic life. The sample is deemed'hazardous if tha Lcso is l 500 mg/L. <br /> This report describes'the procedures used and the results obtained for the does waste <br /> aquatic toxicity meeningtest(s)performed by Block Fn*OD=nW Sa iices.(BES)Bor <br /> Environ, <br /> BF'S is an-Emicvmme l LabOrat*rY Accreditatipn Program certified laboratory(#1 S.12). <br /> 2. MATERIALS AND NIETRODS <br /> 2.1 TEST ORGANIsms <br /> • Rainbow Trout, OncvrhYnchuf myldss,obteined'from a:.commercial supplier. <br /> 2.2 T'PRO. LtRt <br /> A deWW pracedcae for this testis outlined:in laboratn <br /> suw4 ppft-ating prmdum(Sops). <br /> kept at the'RES'laboratory. These SOPS are based upon the foDo .idermaes: <br /> • Califoitlia's Title 22 Code,Section#66261140*, fttie.Acute.Rioassiay <br /> Pracedvre.v fax°8=dr s Waste Samples,7Poia d Miller, 1988.E California <br /> Dap went of Fish and Gme <br /> • G ideiiW far Performing ft&Acute T-bikuy Fish WOOsSaYs ircUunicoal and <br /> Lndustrzaf Wastewaters,'KoppenW, 1976,CzWtniis Dbpattmeat of Fish and Oaf <br /> + &4ndrd Methodsfor the Examinatiqn of Water axed t&Srewater, 191 Edition, <br /> Ainarican Puhlie Health Association, 1995. <br /> 2.3 DATA ANALYSIS <br /> All tOxicity testing results will be analyzed using an appropriate statistical method to dew=jte a <br /> LCSQ arcd the comspandin8 9S%confidence limits. <br /> BES#21'584 Page 2 of 3 <br />