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1455 McDowell Blvd. North, Ste. D <br />KI)Sequoia Petaluma, CA 94954 <br />(707)792-1865 <br />Aw Analytical FAX (707) labs.c 42 <br />www.sequoias.com <br />Sequoia Analytical- Petaluma <br />Client Project ID: P011656-05 Sampled: 11/21/00 <br />1455 N. McDowell Blvd. Ste D <br />Sample Descript: Soil Composite Received: 11/27/00 <br />Petaluma, CA 94954 <br />Analysis Method: See below <br />Attention: Richard Stover <br />Lab Number. W011560-05 Reported: 12/7/00 <br />STATIC HAZARDOUS ABBREVIATED SCREEN BIOASSAY <br />Species: Pirnephales promelas <br />Common Name: Fathead Minnow <br />Min. length: 27 mm <br />Mean length: 29 mm Max. length: 31 mm <br />Min. weight: 0.25 g <br />Mean weight: 0.29 g Max. weight: 0.35 g <br />Dilution Water: Synthetic Softwater <br />Hardness 40-48 Control <br />750 ppm <br />Duplicate 750 ppm <br />Organisms/Tank: 10 <br />Organisms/Conc.: 20 <br />Tank Depth: 13 cm <br />Tank Volume: 10 L <br />Acclimation Temp.: 20 °C <br />Supplier: Sticklebacks Unlimited <br />Alkalinity, mg/L <br />Hardness, mg/L <br />Initial Final <br />Initial Final <br />24 29 <br />41 43 <br />28 36 <br />46 50 <br />28 34 <br />45 51 <br />Initial 24 Hr 48 Hr 72 Hr 96 Hr <br />DATE 11/30/00 1 12/1/00 12/2/00 12/3/00 12/4/00 <br />Dunliratn <br />750 ppm <br />9.0 <br />19 <br />8.0 8.1 <br />18 <br />7.7 <br />0 <br />6.9 <br />18 <br />7.5 <br />0 8.3 <br />18 <br />7.4 <br />0 8.1 <br />18 <br />7.5 <br />0 <br />250 ppm <br />9.2 <br />19 <br />7.9 7.9 <br />18 <br />7.5 <br />0 <br />6.8 <br />18 <br />7.4 <br />0 8.5 <br />18 <br />7.4 <br />0 8.1 <br />18 <br />7.4 <br />0 <br />Pass _x_ Fail <br />Remarks: The screen fails if > 40% of the fish die in the 750 ppm concentration. <br />Analyst: K. Anderson / Method Reference: Static Acute Bioassay Procedures for Hazardous Waste Samples, <br />M. Green November 1988, California Department of Fish and Game WPCL. <br />,ttZUUTAANALTIILAL, UAPff 1L/1 <br />Kyle Anderson <br />Senior Analyst <br />Total <br />Dead <br />i <br />0 <br />0 <br />M00 <br />W01156005YET <1> <br />