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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY Aquatic <br /> Testing <br /> . Laboraiorles <br /> Lab No.: �t7fUd1 <br /> s; <br /> ClientfE D: C <br /> L. 22 <br /> TEST SUMMARY <br /> Species:Pimephrales promelas. Source:In-Lab Culture. <br /> Fish length(mm):av:27;min:26;max:29. Regulations: CCR Title 22. <br /> Fish weight(gm):av: 0.47;min: 0.40;max: 0.59. Test Protocol: California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature:20+/-2°C. Test type:Static. <br /> Aeration: Single bubble through 30 bore tube. Feeding:None. <br /> Number of replicates:2. Number offish per chamber: 10. <br /> Dilution water: Soft reconstituted water(40-48 mg/l CaCO3). Photoperiod: 1618 hrs light/dark. <br /> QA/QC BatchNo.: RT-100803. <br /> TEST DATA <br /> INITIAL 2Q Hr 48 Hr� 72 Hr 96 Hr <br /> Date/Time: - w� �, d r5 t o o <br /> Analyst: <br /> aC DO pH °C DO I pH I #A °C DO I pH I #D °C DO pH WD 7 °C DO pH #D <br /> Control A a . (/ A a <br /> Control B , -� Z Q <br /> 400 mg/l A a 3 y .r7 �`?q S.Q /a� <br /> 400 mg/1 B v%F, F,Y 2 3 0 x 7. � it <br /> 750 mg/l A s •`( Sf 7 Y L' <br /> 750 m 13 ) v.7 `� 8. —7," <br /> Comments: Extraction method. Mechanical shaking _. <br /> None(aqueous solution) -- <br /> Dissolved Oxygen(DO)readings in mg/l 02. <br /> CONTROL HIGH CONCENTRATION Total Number Dead <br /> Alkalinity Hardness Alkalinity Hardness Control /20 <br /> Initial mgn CaCO, mgA CSCO, mgA CaCO, mgn CaCq 400 mg/l /20 <br /> Ffinal 3�- mg/t Caco, �j 3 mgn Caco, Mg11 taco, E 0 mgn taco, 750 m 1 0 120 <br /> RESULTS <br /> the checked result applies based on fish survival rates <br /> PASSED LC50>750 mg/1 (<40%dead in 750 mgli cone.) <br /> FAILED Z40%dead in 750 mg/1 (close to passing-definitive test recommended) <br /> FAILED LC50¢400 mg/1 (>60%dead in 400 mg/1 cone.) <br />