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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY &qua'10 <br /> lasng <br /> LabLaboratories <br /> C1ient1W:e_'4kr*(1k-4A uc# 1 zzX0 <br /> 41 <br /> TEST <br /> TEST SUMMARY <br /> Species:Pimephales promelas. Source:In-Lab Culture. <br /> Fish length(mm):av:27;min: 26;max:29. Regulations:CCR Title 22. <br /> Fish weight(gm): av:0.47;min:0.40;max: 0.59. Test Protocol:California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature:20+/-2°C. Test type: Static. <br /> Aeration: Single bubble through 30 bore tube. Feeding:None. <br /> Number of replicates:2. Number of fish per chamber: 10. <br /> Dilution water: Soft reconstituted water(40-48 mg/1 CaCO3). Photoperiod: 16/8 hrs lightldark. <br /> QA/QC Batch No.:RT-100803. <br /> TEST DATA <br /> INITIAL 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date/Time: evv 1A Woo-] <br /> Analyst: <br /> °C DO pH °C DO pH #D °C DO pH #D11 °C I DO I pH #D °C DO pH #D <br /> Control <br /> Control B <br /> 400mg/l A 0 VldiL,221 O l yo7-1 L <br /> 400 mg/l B VW 92 23�i v `� <br /> 750moA (� �.7 � O d S � g. a 2 <br /> 750 m B �: ✓� .S I <br /> Comments: Extraction method: Mechanical shaking <br /> None(aqueous solution) -4 <br /> Dissolved Oxygen(DO)readings in mall O, <br /> s <br /> s <br /> CONTROL HIGH CONCENTRATION Total Number Dead <br /> - --- i <br /> Alkalinity Hardness Alkalinity Hardness Control /20 <br /> Initial mgrl Cacol mgA Cac% mg/1 Caco) muA CaCO3 400 mg/l () /20 <br /> Final �I.IX man CaCO3 m911 CaCol 3 / mgA CaCO, 11;L me/i CaM 1 750 mg/l /20 <br /> RESULTS <br /> the checked result a Iles based on fish survival rates <br /> i <br /> PASSED LC50>750 mg/1 (<40%dead in 750 mg/l conn) <br /> * FAILED >-40%dead in 750 mg/1 (close to passing-definitive test recommended) <br /> FAILED LC50<400 mg/1 (>60%dead in 400 mall cone.) <br />