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ZALCO LABORATORIES, INC <br /> Analytical and Consulting Services <br /> 4309 Armour Avenue (661) 395-0539 <br /> Bakersfield, CA 93308 FAX (661) 395-3069 <br /> SAMPLE BIOASSAY INFORMATION Lab#: 1903132-2 <br /> [X] 3510 Aquatic Toxicity,96-hour LC50 <br /> Test Type: Screening Flow: Static Tank Volume: 10 liters <br /> Aeration: As Needed Acclimation Temp: 20 C Shaker:Time: <br /> Container:4 Fathead Minnow Species:Pimephales promelas <br /> Source:Thomas Fish Co. Carrier: OnTrac DateRec'd: March 15,2019 <br /> pH adjustment required: Yes [X]No(see pH control worksheet) Avg.Weight: 0--0- g <br /> Methanol carrier solvent required: I Yes [X]No(see MeOH control worksheet) Avg. Length: LA k mm <br /> No.Organisms/Conc.: to Dilution Water:Reconstituted Fresh Water <br /> Hardness Alkalinity EC, <br /> as CaCO3,Mg/l as CaCO3,Mg/I umhos/cm @ 25c <br /> Dilution Water 1� \ I �-o <br /> Initial Control LVL bcj <br /> Final Control 190 <br /> Initial Sample <br /> Final Sample 7- <br /> Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date: 03/18/2019 03/19/2019 03/20/2019 03/21/2019 03/22/2019 <br /> Time: 15:00 15:00 15:00 15:00 15:00 <br /> DO C pH DO 'C PH ft M DO oc PH 9 M DO -C PH UM DO C pH #M #Dead <br /> Control# I s".1 <br /> 400 mg/l <br /> (q <br /> 400 mg/1 • SZ 0 0 <br /> 750 mg/I13 7`0 r) 71D <br /> L o <br /> 750 mg/1 0 C) C"L ag <br /> Comments: <br /> LC50: mg/I <br /> Analyst: Date: <br /> Batch ID: <br />