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FARM SERVICES FIRST PROJECT PLANT DISEASE DIAGNOSTIC SERVICES This is a plant disease testing services for the agricultural growers. We will be testing plants, seeds soil and water samples for plant diseases, for growers,plant nurseries,vegetable farmers, grape growers, almond and other nut tree farmers and seed companies. We don't do research. There are already established methods for disease diagnosis and protocols,we use them.We will be receiving these samples within California State and also from out of California and out of the country. We will obtain permits from California Department of Agriculture(CDFA) and United States Department of Agriculture(USDA)to receive and test seeds,plants,soil and water for diagnosis of plant pathogenic bacterial,fungal,nematodes and viral diseases.This will be a BSL level'II laboratory.We also test E coli, Salmonella on vegetables and water. We will be using conventional methods like plating the samples on semi and selective media and observing them under the microscope, and also use molecular methods for disease diagnostics. In this,we will extract the DNA from the samples and use established protocols to determine the disease. This tests are usually done for plant virus diseases. Grapes has got 17 different virus diseases. Once we diagnose the diseases, we send the report to the respective farmers and we charge them for our diagnostic services anywhere between $100 to $300 per test per sample. The laboratory will be audited by CDFA and USDA. We will be sending bi-annual reports to the Agriculture Commissioner in the County and to CDFA,with details about the samples we received within California and diagnosis. The used and unused samples will be disposed of as per the CDFA protocols (the samples will be autoclaved and then disposed appropriately). Hazardous material if any will be disposed appropriately. SECOND PROJECT TISSUE CULTURE Micro propagation(Tissue culture)is the practice of rapidly multiplying stock plant materials to produce a large number of identical disease free progeny plants. Genetically identical plants are produced from one plant. A single node will produce a shoot within 4-6 weeks that will have 4— 6 nodes.This will be done aseptically in laboratory conditions. Hundreds of thousands of genetically identical plants can be produced from one node. These plant-lets after a certain time in the incubation chamber will be transferred to the growth room, then to shade house for acclimatization and then to greenhouse waiting to further distribution the customers. The target crops are Ornamentals, Strawberries, artichoke, coffee, almonds,walnuts,peaches and much more. I have a PhD in Plant Pathology and 35 years' experience in plant disease diagnostics. We will have full-time trained qualified laboratory technicians, office manager and an accountant. We also will have a greenhouse at the side of the building Our lab will be audited by the County State Fund, USDA and CDFA for compliance.