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Pacific EcoRisk Environmental Consulting and Testing <br /> 1. INTRODUCTION <br /> GeoAnalytical Laboratories, Inc. (GeoAnalytical)has contracted Pacific EcoRisk(PER)to <br /> conduct a screening-level California Title 22 hazardous waste acute toxicity evaluation of a <br /> waste sample designated S-2,S-3,S-4,S-4@2' Composite. This evaluation consisted of <br /> performing a 96-hour acute toxicity test with juvenile fathead minnows (Pimephales promelas). <br /> This report describes the performance and results of this test. <br /> 2. TOXICITY TEST PROCEDURES <br /> This testing followed the guidelines established by the California Dept. of Fish&Game manual <br /> "Static Acute Bioassay Procedures for Hazardous Waste Samples" (Polisini and Miller 1988), <br /> with additional guidelines as provided by the California Dept. of Health Services (Janna White, <br /> personal communication). <br /> 2.1 Sample Receipt and Handling <br /> The waste sample designated S-2,S-3,S-4,S-4@2' Composite was delivered to the PER testing <br /> laboratory on August 26, 2020,where it was placed under refrigeration at 4°C until being used to <br /> prepare the test solutions. The chain-of-custody record for the collection and delivery of this <br /> waste sample is provided in Appendix A. <br /> 2.2 Preparation of the Test Media <br /> The Lab Water Control medium for this test consisted of synthetic US EPA soft water, prepared <br /> by adding reagent grade salts to Type 1 lab water(reverse-osmosis de-ionized water). The waste <br /> sample was homogenized, after which sub-samples of waste were mixed with the Lab Water <br /> Control medium for six hours,using a rotary mixer. After mixing, these preliminary solutions <br /> were used to prepare test solutions at sample concentrations of 250, 500, and 750 mg/L. <br /> 2.3 Acute Toxicity Testing with Fathead Minnows <br /> The fathead minnows used in this test were obtained from a commercial supplier(Thomas Fish <br /> Company, Anderson, CA). These fish were maintained in aerated aquaria containing 4 ppt <br /> salinity water at 20'C prior to their use in this test. <br /> This hazardous waste test was run at sample concentrations of 250, 500, and 750 mg/L, with a <br /> Lab Water Control treatment consisting of the same synthetic EPA soft water. There were two <br /> replicates at each test treatment, each replicate consisting of 9.2 L of media in a 10-L container. <br /> Water quality characteristics(pH and dissolved oxygen [D.O.])were determined for the test <br /> solution in each test replicate prior to initiation of this test. <br /> Page 1 L))> <br /> 4/13 (((( <br />