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Packaging: Package the instruments with hinges in the open position to ensure that the ridges and <br />crevices of the instruments are sterilized. <br />Loading: Load the autoclave with the packages upright on their sides. Peel packs should be on <br />edge with the plastic side next to a paper side to allow for steam penetration. Do not overload the <br />autoclave to allow proper flow of the steam to achieve sterilization. <br />Steam Sterilization: Temperature should be 121'C or 250° F; pressure should be 106kPa <br />(15lbs/in2); 30 minutes for packaged items. At a higher temperature of 132° C or 279° F, pressure <br />should be 30 lbs/int; 15 minutes for packaged items. <br />Allow all items to dry before removing them from the autoclave. Use clean gloves to handle <br />packaged items. <br />Pressure settings (kPa or lbs/int) may vary slightly depending on the autoclave used. Follow <br />manufacturer's recommendations for your autoclave. <br />Exposure time begins only after the autoclave has reached the target temperature. <br />Source: Adopted from Principles and Methods of Sterilization in Health Sciences. <br />JJ Perkins. 1983 <br />Page 11 of 12 <br />