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intrastate rivers and streams, as well as their intermittent tributaries. The limit of federal jurisdiction of Non -Tidal Waters of the U.S. extends to the "ordinary high water mark". The ordinary high water mark is established by physical characteristics such as a natural water line impressed on the bank, presence of shelves, destruction of terrestrial vegetation, or the presence of litter and debris. Jurisdictional wetlands are vegetated areas that meet specific vegetation, soil, and hydrologic criteria defined by the ACOE Wetlands Delineation Manual and Regional Supplement (ACOE, 1987; 2008). Jurisdictional wetlands are usually adjacent to or hydrologically associated with Waters of the U.S. Isolated wetlands are outside federal jurisdiction, but may be regulated by RWQCB under the State Wetlands Program. No potentially jurisdictional wetlands or Waters of the U.S. were observed in the site. The site consists of upland grassland vegetation that is periodically disked and soils appear well draining. There are no aquatic features mapped in the site in the National Wetland Inventory (NWI) (Attachment D). While it may be best described as a "ditch", Bieghle Drain is a potentially jurisdictional Waters of the U.S. Bieghle Drain is adjacent to the north edge of the site, is straight, trapezoidal in shape, and contains sparse amounts of hydrophytic vegetation. The potential jurisdictional limit of the drain is defined by an ordinary high water mark (OHWM). The drain carries water from east to west. Bieghle Drain is depicted as a "blue -line" stream on the USGS topographic map (Figure 2) and as a Riverine feature in the National Wetland Inventory (NWI) (Attachment D). Drainage patterns apparent on historical topographic maps and aerial photographs suggest the drain is a realigned ephemeral creek that flowed in to Duck Creek further west of the site. While some sections of Bieghle Drain to the west of the site have been piped, the drain is still tributary to Duck Creek. 2706 Pock Lane: Biology 10 November 23, 2021