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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY TL Aquatic <br /> Testing <br /> Lab No.: A 1 g o,9Zy t 1 -c-o / Laboratories <br /> Client/ID: Tvq +•1 Y o - 2 tf S r 6-D-f <br /> TEST SUMMARY <br /> Species:Pimephales promelas. Source: 11jgej�e3 X%A . <br /> Fish weight(gm): av: *_28 ; min: D.;L ; max: f7j a . Regulations: CCR Title 22. <br /> Reference Toxicant: SDS conducted monthly per source. Test Protocol: California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature: 20+/-2°C. Test type: Static. <br /> Aeration: none/minimum (>4.0 mg/1 DO). Feeding:None. <br /> Number of replicates: 2. Number offish per chamber: to <br /> Dilution water: Soft reconstituted water(40-48 mg/l CaCO,). Photoperiod: 16/8 hrs light/dark. <br /> TEST DATA <br /> INITIAL 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date/Time: 8.21-t It o)o Q•2 2-t t_01_, 9-2 3-[ <br /> Analyst: _ -_ I <br /> °C I DO I pH °C I DO I pH I # D 1 DO I pH I #D °C I DO I pH I #D °C I DO I pH I #D <br /> Control A to.( 7.6 77.7 D b. •O . 7 0 1 760 2,._1 0 <br /> Control B 28• 7• ' l 7 6 a, 0 . d 0 ° 017, o 7., 7 V <br /> 400 mg/l A t. Z•,1 7-r O 2+• I 7j 7- 1 0 ZA 7. 7 O 20.2 0 7.1 0 <br /> 400 mg/l B 2•. s. Ica &J -/-117. 7 0 7- 2 7. 1 0 . �A 2 7-o 7. 7 U 6. 0 1 7.0 C) <br /> 750 mg/I A lo. 8 3 „1 26.3 7. 7 7•7 v 2o.f 7 1 77 0 ?.-.1 1.0 7.6d 2t Z L•I 7.9 6 <br /> 750 mg/l B ?o. p. 4. 20.2 7. 7.6 O KV , 17.0 . Q �.2 7. 1 7. 6 20.1 6.0 7. 7 O <br /> Comments: Extraction method: Mechanical shaking V11-1, Dissolved Oxygen(DO)readings in mg/1 OZ. <br /> None(aqueous solution).-A64_. <br /> Test Aeration: None / . <br /> * Aerated_4. (Minimum needed to maintain DO>5.5 mg/L through narrow-bore glass tube at<100 bubbles per minute) <br /> CONTROL HIGH CONCENTRATION Total Number Dead <br /> Alkalinity Hardness Alkalinity Hardness Control C> / 1,,0 <br /> Iriltlal '3> m9/1 CaCO3 S mg/l CaCO, 33 mg/l CaCO, N mg/1 CaCO, 400 mg/l G / 2-0 <br /> Final 3 mgA CaCO, mg/l CaCO, );I mg/l Caw, Y mg/l CaCO, it 1, 750 mg/1 1 Q / V <br /> RESULTS <br /> the checked ✓ result applies based on fish survival rates of this test; NA- notapplicable) <br /> PASSED LC50>750 mg/1 (<40%dead in 750 mg/l conc.) <br /> FAILED z40% dead in 750 mg/l (close to passing- definitive test recommended) <br /> y/} I FAILED LC50<400 mg/l (>60%dead in 400 mg/l conc.) <br /> Page 29 of 32 8/30/2019 <br />