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i Spore Test Checklist <br />❑ Verified 3M Attest Auto Reader is plugged in for at least 30 minutes prior to start of test <br />❑ Obtain biological indicators <br />❑ Don PPE <br />❑ Verified test pack and control tubes are from the same manufacturer <br />❑ Verified all biological indicator tubes are not expire <br />❑ Test pack loaded in the center of the medical waste load <br />❑ Placed the "Test in Progress, Do Not Operate" sign on the chamber <br />❑ Ran sterilization cycle, verifed sterilization cycle at 250degrees F for 30 minutes <br />❑ Load was cooled and test pack was removed wearing PPE <br />❑ The medical waste was left in the chamber <br />❑ The incubation/readout procedure was followed: <br />Don PPE <br />Open test pack and remove indicator tube <br />Close the biological cap by pressing down <br />Crush the glass tube in the crushing well <br />vial <br />Place the tube into the incubator well <br />Cover and close, wait for results (at least 3 hours) <br />❑ Results read <br />Negative results: Control tube will be red, sterilized tube will be green <br />Positive results: Control tube will be red, sterilized tube will be red <br />Positive results: Control tube will be green, sterilized tube will be red <br />Positive results: Control tube will be green, sterilized tube will be green <br />❑ Test is negative; documented all information in the Record Log Book, including: <br />Date <br />Load number <br />Sterilizer number <br />Load contents <br />Tube Lot number/expiration date <br />Time and initials <br />Chemical indicator <br />Incubator well numbers, time in/time out, initials <br />Check "131" box <br />positive or negative under Visual <br />❑ Test is negative; Notified EVS can use the Sanipak and removed sign <br />Test is positive; test will be repeated three times, in the event the test is still positive, <br />repairs will be scheudled and the San! -Pak will be taken out of service until three <br />❑ additional tests result in negative tests. <br />❑ Test is positive; notify EH&S Manager, Facility Services Director, Chief Engineer <br />and EVS Manager <br />Performed by: <br />Title: <br />Verified by: Title: <br />Date: <br />Date: <br />Sani-Pak Spore Testing Checklist EC.SA.081A (Attachment) <br />February 2009 <br />