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Sterilization Procedures 6. A body art facility that does not afford access to a decontamination and sterilization area that meets the standards of subdivision (c) of Section 119314 of the California Health and Safety Code or that does not have sterilization equipment shall use only purchased disposable, single -use, pre -sterilized instruments. In place of the requirements for maintaining sterilization records, the following records shall be kept and maintained for a minimum of 90 days following the use of the instruments at the site of practice for the purpose of verifying the use of disposable, single -use, pre -sterilized instruments: • A record of purchase and use of all single -use instruments. • A log of all procedure, including the names of the practitioner and client and the date of the procedure. Operating Conditions for Autoclave Cleaning: Remove all material on the instruments during the cleaning process to ensure that the sterilization process is achieved. The cleaning process can be a manual cleaning or by use of an ultrasonic machine. Packaging: Package the instruments with hinges in the open position to ensure that the ridges and crevices of the instruments are sterilized. Loading: Load the autoclave with the packages upright on their sides. Peel packs should be on edge with the plastic side next to a paper side to allow for steam penetration. Do not overload the autoclave to allow proper flow of the steam to achieve sterilization. Steam Sterilization: Temperature should be 1210 C or 250° F: pressure should be 106kPa (15lbs/in2); 30 minutes for packaged items. At a higher temperature of 1320 C or 279° F, pressure should be 30 Ibs/int; 15 minutes for packaged items. Allow all items to dry before removing them from the autoclave. Use clean gloves to handle packaged items. Pressure settings (kPa or Ibs/in2) may vary slightly depending on the autoclave used. Follow manufacturer's recommendations for your autoclave. Exposure time begins only after the autoclave has reached the target temperature. Source: Adopted from Principles and Methods of Sterilization in Health Sciences. JJ Perkins. 1983 Page 10 of 11