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Encro • ia/insi9 hts The QuantArray°-Chlor Approach Quantification of Dehalococcoides, the only known bacterial group capable of complete reductive dechlorination of PCE and TCE to ethene, has become an indispensable component of assessment, remedy selection, and performance monitoring at sites impacted by chlorinated solvents. While undeniably a key group of halorespiring bacteria, Dehalococcoides are not the only bacteria of interest in the subsurface because reductive dechlorination is not the only potential biodegradation pathway operative at contaminated sites, and chlorinated ethenes are not always the primary contaminants of concern. The QuantArray®-Chlor not only includes a variety of halorespiring bacteria (Dehalococcoides, Dehalobacter, Dehalogenimonas, etc.) to assess the potential for reductive dechlorination of chloroethenes, chloroethanes, chlorobenzenes, chlorophenols, and chloroform, but also provides quantification of functional genes involved in aerobic (co)metabolic pathways for biodegradation of chlorinated solvents and even competing biological processes. Thus, the QuantArray®-Chlor will give site managers the ability to simultaneously yet economically evaluate the potential for biodegradation of a spectrum of common chlorinated contaminants through a multitude of anaerobic and aerobic(co)metabolic pathways to give a much more clear and comprehensive view of contaminant biodegradation. The QuantArray-Chlor is used to quantify specific microorganisms and functional genes to evaluate the following: Quantification of important halorespiring bacteria(e.g. Dehalococcoides, Anaerobic Dehalobacter,Dehalogenimonas,Desulfitobacterium spp.) and key functional Reductive genes(e.g. vinyl chloride reductases,TCE reductase,chloroform reduc- • • tase) responsible for reductive dechlorination of a broad spectrum of chlorinated solvents. Several different types of bacteria including methanotrophs and some toluene/phenol utilizing bacteria can co-oxidize TCE,DCE,and vinyl Aerobic • • • chloride. The QuantArray°-Chlor quantifies functional genes like soluble methane monooxygenase encoding enzymes capable of co-oxidation of chlorinated ethenes. Ethene oxidizing bacteria are capable of cometabolism of vinyl chloride. Aerobic ( • • In some cases,ethenotrophs can also utilize vinyl chloride as a growth of Vinyl • • supporting substrate. The QuantArray®-Chlor targets key functional genes in ethene metabolism. How do QuantArrayso work? The QuantArray®-Chlor in many respects is a hybrid technology combining the highly parallel detection of mi- croarrays with the accurate and precise quantification provided by gPCR into a single platform. The key to highly parallel gPCR reactions is the nanoliter fluidics platform for low volume, solution phase gPCR reactions. 1 10515 Research Drive Knoxville,TN 37932 Phone: 865.573.8188 Fax: 865.573.8133 Web: www.microbe.com