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Pacific EcoRisk Environmental Consulting & Testing <br />1. INTRODUCTION <br />Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical), Pacific EcoRisk (PER) <br />conducted a screening level Title 22 "Hazardous Waste" fish toxicity evaluation of a liquid waste <br />sample designated "Liquid -I". This toxicity test consisted of a 96 -hr static bioassay with juvenile <br />fathead minnows (Pimephales promelas). This report describes the performance and results of <br />this test. <br />2. ACUTE TOXICITY TEST <br />The methods used in conducting this test followed the guidelines established by the California <br />Dept. of Fish & Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br />(Polisini and Miller 1988), with additional guidelines as provided by the California Dept. of <br />Health Services (Janna White, personal communication). <br />2.1 Receipt and Handling of the Waste Sample <br />A liquid waste sample designated "Liquid -1" was delivered to the PER testing laboratory on <br />April 22, 2014, where it was placed under refrigeration at 4°C until being used to prepare the test <br />solutions. The chain -of -custody record for the collection and delivery of this waste sample is <br />provided in Appendix A. <br />2.2 Preparation of the Test Media <br />Sub -samples of the waste were mixed with the Lab Water Control medium for 6 hrs, using a <br />rotary mixer; the sample was homogenized as much as possible before mixing. The Lab Water <br />Control medium consisted of synthetic US EPA "soft' water (prepared by adding reagent grade <br />salts to Type I lab water [reverse -osmosis de -ionized water]). After mixing, these preliminary <br />solutions were used to prepare test solutions at sample concentrations of 250, 500, and 750 mg/L. <br />2.3 Acute Toxicity Testing with Fathead Minnows <br />The fathead minnows used in this test were obtained from a commercial supplier (Thomas Fish <br />Company, Anderson, CA). These fish were maintained at 20°C in aerated aquaria containing <br />EPA synthetic soft water prior to their use in this test. <br />This hazardous waste evaluation was run at sample concentrations of 250, 500, and 750 mg/L, <br />with a Lab Water Control consisting of the same synthetic EPA "soft' water. There were 2 <br />replicates at each test treatment, each replicate consisting of 9.2 L of media in a 10-L container. <br />Water quality characteristics (pH and dissolved oxygen [D.O.]) were determined for the test <br />solution in each test replicate prior to initiation of this test. <br />Page 1 L> <br />4113 <br />