Laserfiche WebLink
Pacific EcoRisk <br />Environmental Consulting & Testing <br />The test was initiated with the random allocation of 10 juvenile fathead minnows into each <br />replicate container. These containers were placed in a temperature -controlled room at 20°C, <br />under fluorescent lighting on a 16L:8D photoperiod. <br />Each day, each replicate container was examined, and the number of live fish in each was <br />recorded. Routine water quality characteristics (temperature, pH, and D.O.) of the treatment <br />waters were measured and recorded for each replicate container each day. <br />After 96 (±2) hrs, the test was terminated and the number of live fish in each replicate container <br />was determined. The fish in a randomly selected Lab Control treatment replicate were then <br />cuthanized and measured for total length (to the nearest 0.1 mm) and weighed (to the nearest <br />0.001 g). The resulting survival data for the waste treatments were evaluated to determine the <br />LC5o using the CETIS®statistical software (TidePool Scientific, McKinleyville, CA). <br />3. RESULTS <br />3.1 Effects of the Waste Sample on Fathead Minnows <br />The results of this test are summarized in Table 1. The LC50 of this sample was >750 mg/L, <br />which is greater than the Title 22 "Hazardous Waste" classification threshold of 500 mg/L; <br />accordingly, this sample should not be considered "hazardous" with respect to fish toxicity. The <br />test data and summary of statistics for this test are presented in Appendix B. <br />Table 1. Results of the hazardous waste toxicity evaluation of the waste sample "Liquid -1". <br />Waste Sample Treatment Level <br />Mean % Survival <br />Lab Water Control <br />95 <br />250 mg/L <br />95 <br />500 mg/L <br />95 <br />750 mg/L <br />95 <br />Summary of.Key Statistics <br />LC so = <br />>750 mg/L <br />Page 2 f f ) <br />5/13 l <br />