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microbial activity was such that we felt that, with the addition of the horse manure <br /> compost, a sufficient rate of detoxification could be achieved and eventual breakdown of <br /> diesel material would be obtained <br /> For some time, there has been knowledge that many common soil bacteria possess the <br /> ability to metabolically shaft to take advantage of fuel hydrocarbons as food when <br /> encountered in soil. Frequently, the bacteria involved include many different species of <br /> pseudomonas Some more recent work has indicated the probability that many types of <br /> fungi are extremely active in the bio-degradation process and may actually present <br /> dominant roles in mineralizing fuel hydrocarbons. There was no attempt made in this <br /> investigation to determine what the particular strains of microorganisms were. Although <br /> many organisms have been identified as active In the degradation of petroleums <br /> hydrocarbons, most of them are Imown as opportunistic utdazers. <br /> The opportunistic utilizer is simply an organism which is able to use more than one type <br /> of food and has the ability to shift its diet to whatever food source is most prevalent, i e , <br /> a carbon source. In this case, the organisms of interest were those adapted to utilizing <br /> petroleum hydrocarbons as a primary food (carbon) source. 1nl-some situs ons,� am <br /> inoculum may <br /> wprove;totrbe! 3ieneficial; hdweveg;x�theadditioiiEofa ;foreign sourceof� <br /> organ�smmay�uublt?the rit�yof at~gra�dationirmatuig competiti4iivfo�resoicsvintbi <br /> f e �iatrc ,tin andigeiious`pope a#non. # 0bu� � o;b public �i la#i a$ api bbl �m <br /> assaca e�.d�vi ib�usiitg,ais additional inoculum onihicrnorganism3i11 the�saz;�1as;therre��tnar <br /> • bei afeaz tnatt]its aid'c�����croorganism�imigli :lie7uncontrollabie: In araost cases, the <br /> native population and character of microorganisms is sufficient using the bioremediation <br /> method under discussion. In the case under discussion, no exotic inoculum or preparation <br /> was involved The native soil was found to contain a sufficient population of the desired <br /> microorganisms. <br /> This bioremediation process operates by accelerating a naturally occurring process which <br /> is always taking place in the soil, however, of the natural process were allowed to continue <br /> unaided, many years would be required to break down the diesel Alteration of the soil <br /> environment is necessary to provide the conditions necessary for the maximum <br /> mineralization of the contaminants. We found that the soil to be treated was literally <br /> loaded with microorganisms. The crux of successful bioremediation is to promote the <br /> welfare of the maxed inhabitants of the soil, the microorganisms present in the ecosystem <br /> as a whole. Providing an ideal growth environment produces the rapid-rate bio- <br /> degradation necessary to economically remediate diesel-contaminated soil <br /> This particular operation utilized the organisms in the soil which exist in a natural state <br /> of equilibrium This equilibrium hinges upon the presence of different food sources, the <br /> availability of nutrients, the presence of oxygen and moisture, and other factors. Certain <br /> of these factors are always in short supply, thereby keeping a check on the populations <br /> of the microorganisms We enhanced the utilization of the organisms by aligning the <br /> eating habits of the microorganism populations to include fuel hydrocaibons and by using <br /> the horse manure compost additive a., a supporting nutrient In so doing, limiting factors <br />