Laserfiche WebLink
z <br /> y �• <br />!=c'n <br /> Issued: 8/98/87 <br /> MICRO SOP-P19 <br /> CENTRAL RESEARCH AND DEVELOPMENT DEPARTMENT APPENDIX Kc <br /> HASRELL LABORATORY FOR TOXICOLOGY <br /> AND INDUSTRIAL MEDICINE <br /> Environmental Toxicology and <br /> Information Services Section <br /> Microbiology Procedure SDP-P19 <br /> SERIAL DILUTION SPREAD PLATE TECHNIQUE <br /> (HETEROTROPHIC PLATE COUNT) <br /> Y <br /> Purpose - <br /> This SOP des.•,Jbes a serial dilution spread plate procedure for <br /> enumerating viable aerobic and facultatively anaerobic heterotrophic bacteria <br /> in an aqueous sample. <br /> s Other Relevant SOP's <br /> o Microbiology Procedure SUP-PO5 Culture Media Preparation <br /> o .Microbiology Equipment SOP-Ell Castle/Sybron Laboratory Sterilizer <br /> (Autoclave) Operation <br /> Responsibility <br /> It is the responsibility of the person performing the Heterotrophic Plate <br /> Count Procedure to follow this SOP. <br /> Equipment <br /> a Biological Safety Cabinet <br /> u Appropriate disinfectant (i.e., Sporicidin) <br /> r `r o lion air incubator (such as Hotpock Progrm:med Refrigerated Incubator or <br /> x equivalent) <br /> o Mater beth for tempering agar (set at 44-50°C) <br /> Y 4 <br /> o Vortex Mixer (ouch as the Vortex-Genie, Fisher Scientific, catalog <br /> 312-812 or equivalent) <br /> - ---— o Colony counter with automatic tally, if <br /> , a <br /> Mom <br /> f <br /> r _ <br />