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f <br /> Issued! 8/18/87 <br /> MICRO SOP-P19 <br /> o Autoclave <br /> o Pipet containers of stainless steel, aluminum, plastic or glass for <br /> contaminated pipets <br /> o Sterile 100 mm x 15 gran petri diahes (preferably plastic) <br /> o Bunsen/Fisher gas burner or alcohol lamp <br /> o Sterile 1.0 mL pipets, glass or plastic <br /> o Pipetman* automatic pipet or equivalent (200 uL with sterile tips) <br /> o Bent glass rods ("hockey sticks") in glass, ethanol containing beaker <br /> with lid <br /> o Petri Dish Turntable (Fisher Scientific, catalog 908758) <br /> E' c Pipet filler (VWR Scientific, cat# 53497-009 or equivalent) <br /> o Sterile 16 mm x 150 mm test tubes with plastic caps <br /> F <br /> _Safety Equipment <br /> ',......... o Biological Safety Cabinet <br /> o Blue lab coat <br /> o Safety glasses <br /> o Appropriate gloves for autoclave and disinfectant use <br /> Safety Considerations <br /> o Follow manufacturer's precautions while preparing or using any <br /> disinfectant solution. Consult the Material Safety Data Sheet (MSDS) <br /> for proper use, protection and disposal of the disinfectant. <br /> o Consult the Stine-Haskell Safety and Occupational Health Manuel, <br /> Section I1, Subpart 9 "Procedures for Waste Disposal" and the <br /> Environmental Toxicology's addendum "Section Specific Supplement for <br /> Handling Wastes from the Environmental Biology Section" for details of <br /> required microbiological waste disposal practices. <br /> Media and Reagents <br /> o Trypticasea Soy Agor (TSA, BBLa) plates, or other appropriate medium <br /> -_-_--- -----------o--Sterile-9.0-mL-and_9.9-mL..Phosphate-.Buffer."blanks"--(Standard-Methods,------ ----- -----------_— <br /> x. 1985) in 16 nm x 150.mm capped test tubes <br /> 2 - <br /> f <br />