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—k..7ef -eat <br /> WA <br /> Issued: 8/18/87 lasueds 8/10/07 <br /> MICRO SOP-P19 MICRO SOP-P19 <br /> ID DEVELOPMENT DEPARTMENT A"ENDIX Kc <br /> CRY FOR TOXICOLOGY <br /> MEDICINE <br /> )lsatic or glass for <br /> plastic) <br /> Environmental Toxicology and <br /> Information Services Section <br /> Microbiology Procedure SOP-P19 <br /> SERIAL DILUTION SPREAD PLATE TECHNIQUE with sterile tips) <br /> (HETCROTROPHIC PLATE COUNT) anal containing beaker <br /> T 008758) <br /> ?lbes a aerial dilution spread plate procedure for equivalent) <br /> ?aerobic and facultatively anaerobic heterotrophic bacteria .Bps <br /> 'Le. <br /> 'yr Pr-dura SOP-PO5 Culture Media Preparation <br /> a <br /> 'y Equipment SOP-Ell Castle/Sybron Laboratory Sterilizer <br /> (Autoclave) Operation <br /> I <br /> it use <br /> `bnsibility of the person performing the Heterotrophic Plate <br /> follow this SOP. <br /> 1 ng or using any <br /> i Fety Data Sheet (MSDS) <br /> Safety Cabinet disinfectant. <br /> disinfectant (i.e., Sporicidin) al Health Manual, <br /> posa1 and the <br /> �ubator (such so Notpack Programed Refrigerated Incubator or ecific <br /> aSupplemtsnt for <br /> p . g g Section" for details of <br /> as. <br /> ;for tempering agar (set at 44-50°C) <br /> ' r (such as the Vortex-Genie, Fisher Scientific, catalog <br /> equivalent) er appropriate medium <br /> r <br /> ,ter with automatic tally, if available 'sks" (standard Methods, <br />