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IN E 1 0 <br /> 1 , <br /> 7 <br /> s , <br /> r � <br /> 28 February 1997 <br /> AGE-NC Project No 95-0144 <br /> Page 17 of 20 <br /> 1 � - <br /> degrade hydrocarbons and spread laterally (and vertically) by migration and reproduction <br /> Hydrocarbon-degrading microbes are able to metabolize hydrocarbons and assimilate the resulting <br /> organic chemicals into biomass for reproduction and growth Typically, the light-end hydrocarbons <br /> are degraded first, followed by heavier compounds <br /> 6 3 2 NUTRIENT REQUIREMENTS AND TOXICITY <br /> ' fThe microbes require certain essential nutrients that are usually available in ground water Jf <br /> supplemental nutrients are required, such as nitrogen and phosphorus as ammonium phosphate in <br /> liquid phase,the quantity is usually very low However, a Waste Discharge Permit is required by the <br /> CVRWQCB for the infection of nutrients into ground water <br /> Typically, the microbes utilized in bioremediation are aerobic and usually require additional oxygen' <br /> ' to supplement dissolved oxygen concentrations in ground water Generally, several air spargmg or <br /> in-well aeration sources are sufficient to provide the necessary oxygen However, in some cases the <br /> addition of hydrogen peroxide is necessary <br /> 1 6 3 3 MONITORING ACTIVITIES <br /> 1 <br /> After inoculation,the inoculation wells and ground water monitoring wells should be monitored for <br /> several months by periodic field measurements of organic vapor,'carbon dioxide, and oxygen - <br /> Groundwater samples should be collected and analyzed for petroleum hydrocarbons, bacterial <br /> populations and nutrient availability If monitoring indicates areas of slow remediation, additional <br /> inoculations may be required <br /> 1 ' <br /> 6 3 4 FEASIBILITY STUDIES <br /> Results of the bioremediation pilot tests at other sites indicate that lighter petroleum hydrocarbons, <br /> such as gasoline compounds are biodegraded more readily than longer-chain(heavier)hydrocarbons, <br /> such as diesel fuel compounds Feasibility studies have not been performed at the subject site <br /> 6 3 5 DURATION <br /> - ' <br /> Cleanup durations for in-situ bioremediation will vary,depending upon specific site characteristics <br /> However, an 18 to 36 month treatment period is generally achievable Since dissolved hydrocarbon <br /> , <br /> , 1 r <br /> 1 i <br />