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Geweke Pilot Study 2 - 1 March 194 , Purpose: Ig methods, detect activity of bacteria in the vadose zone by counring 1. Using monitori original bacteria, detecting and counting inoculated bacteria, and monitoring for by- products of the inoculated bacteria. 2, Using monitoring methods, determine whether ground water has been impacted by the bacteria inoculated into the vadose zone. 3. Using the data from the pilot study, draw conclusions about the effectiveness of the remediation system in the vadose zone and potentially in the ground water and make recommendations to improve distribution of bacteria. should 4. Using the data from the pilot study, determine whether the full-blownperiod, p ementationeof implemented. If no change is detected within the 60 to 90 day p the full-blown insim bioremediation system should be reconsidered. GROUND WATER MONITORING FOR THE PILOT STUDY The ground water monitoring program has been divided into fore ee bacterial analysis willbe ns will program determine which program will be implemented. All samples collected before purging. Initially, monitoring will follow Program A. Program A t to detect an impact on ground water caused y Under Program A, monitoring will attempls closest inoculation of bacteria in the vadose the twoel inoculatedonitoring bacteria.nIt was inoculation. MW4 was the only ground water monitoring inoculated to a depth of 40 feet by placing a packer at that depth. MW4 is about 60 feet deep and depth to ground water is also about 60 feet. MW4 has some water in it and a sample may be collected if the well is not purged. If inoculated bacteria reach the ground water, it is likely to occur in MW4 first. MW14, which was not inoculated, is close to both MW4 and VWS, and seems to be the next likely candidate to show the presence of inoculated bacteria. Monitoring Points: MW4 and MW14 Constituents: DO, CO2, bacterial counts and composition Sample Frequency: Mondays and Thursdays As long as no changes in bacterial count and composition and no breakdown products are detected, monitoring will continue under Program A. If changes are detected in the monitored constituents, monitoring will then move to Program B or C.