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s • <br /> 8) Total nitrogen is 33 ppm and phosphate is 11 ppm, as analyzed by AAS. Mass <br /> balance calculations for a [theoretical] plume 100 feet in length, 45 feet in width and <br /> 55 feet in depth, with an average hydrocarbon concentration of 1,000 ppm, and <br /> assuming a porosity of 19% (a very low estimate for medium- to fine-grained sand <br /> with silt), indicates that 41.7 pounds of nitrogen, 2.08 pounds of phosphate and less <br /> that 1 CFM of oxygen will be needed to convert 350 gallons of existing hydrocarbon <br /> into biomass. Currently at the site, there is 1,100 pounds of naturally occurring <br /> nitrogen (26 times more than required) and 367 pounds of naturally occurring <br /> phosphate (more than 176 times more than required). It should also be noted that <br /> both aerobic and anaerobic bacteria will be used in the inoculation(s), decreasing the <br /> importance of oxygen concentration in the soil. <br /> 9) The "radius of influence" of the bacteria is a function of soil type, hydrocarbon type <br /> and, most importantly, time. Theoretically, a single inoculation well within a plume <br /> would be sufficient for motile hydrocarbon-degrading bacteria to remediate the <br /> plume, given sufficient time. However, by increasing the inoculation-well density, <br /> larger numbers of bacteria are introduced and better dispersed within the plume, <br /> allowing for faster remediation. The"optimum"well spacing is thus dependant on the <br /> rate at which remediation is desired. Until such time as at least limited inoculation <br /> has been permitted, no site-specific data on the rate of bacterial migration and <br /> hydrocarbon mitigation can be obtained. <br /> The .hydrocarbons at this site are primarily gasoline and gasoline-breakdown <br /> products. The soil type is primarily medium- to fine-grained sand with some silt. Our <br /> review of existing literature and discussions with microbiologists lead us to conclude <br /> that motile bacteria, under optimal conditions, are able to migrate in excess of 15 <br /> feet within three to four weeks in medium- and fine-grained sands. Soils with a <br /> greater clay content and heavier hydrocarbons (diesel) would require a greater <br /> inoculation volume and well density. For example, in field tests conducted in 1989 <br /> and 1992 near Santa Barbara, California and Conway, Texas, bacteria migrated <br /> fifteen (15) feet through sand and five (5) feet through clay during the first three (3) <br /> months of remediation (James Hoeltgen,.Personal Communication; 1993). <br /> Inoculation wells at this site are located on approximately 15-foot centers. At the <br /> present time, we believe that this well density is appropriate for a one to two year <br /> remediation period. This estimate will be reviewed frequently during the early stages <br /> of remediation as monitoring data become available, and additional wells and/or <br /> monitoring points can be installed to accelerate the remediation as needed to <br /> maintain this schedule. <br /> 10) GeoAudit recommends the following additions to the existing monitoring program: <br /> A. Soil Borings (vicinity of inoculation wells; dates are approximate and based <br /> upon an inoculation date of February 1, 1994): <br /> 3 <br /> a..inxawn.n <br />