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The growth media consists of hospital-grade soy and casein <br /> broth supplemented with food grade sugars. Species present are <br /> as follows: <br /> Genus Species Motile Aerobe Temp. <br /> Range <br /> Bacillus <br /> B . cereus M FA 10-45 <br /> B . Subtilus M FA 5-55 <br /> B. megaterium M FA 3-55 <br /> B. Polymyxa M FA 5-45 <br /> B . macerans M FA 5-50 <br /> Pseudomonas <br /> P. stutzeri M OA 30-41 <br /> P, putida M OA 4-41 <br /> P. rubescens M OA <br /> P. aureofaciens M OA 4-35 <br /> P. aeruginosa M OA 32-41 <br /> Serratia <br /> S marcesans M OA <br /> Azotobacter <br /> A. vinelandii M FA <br /> A. chroococcum M FA <br /> A. paspali <br /> Streptomyces sp. <br /> Rhyzobium sp. <br /> 10. Each well will receive two well volumes of inoculum. For <br /> example, a 4" by 55-foot well will get: <br /> (4 x 4 x 0 . 0408 x 55) (2) gallons = 72 gallons <br /> For purposes of this bioremediation process, the same volume <br /> will be used for 2-inch and 6-inch diameter wells. Therefore <br /> a total of 432 gallons of inoculum will be placed into the <br /> monitoring wells; a total of 648 gallons will be placed into <br /> the remediation wells. However, if new data or information is <br /> obtained these volumes may be adjusted. Because the growth <br /> media has been entirely consumed at the time of inoculation, <br /> no media will be introduced into any well. <br /> 11) At this time only one inoculation is planned. If for some <br /> unforeseen reason a second inoculation is needed, it will be <br /> proposed according to proper regulatory guidelines. <br /> 12) our microbiologist has used mass-balance equations to <br /> determine the amount of various elements that are needed for <br /> bacterial growth. Based on the volume of contaminated soil and <br /> 4 <br />