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0 <br /> 13 December 1995 <br /> AGE-NC Project No. 95-0109 <br /> Page 4 of 8 <br /> which likely accounts for the low percentage of biodegrading organisms detected. A recommendation <br /> for nutrient supplementation to target areas to supplement the observed loss was suggested by the <br /> microbiologist. <br /> Samples will be collected from monitoring wells MW-8, MW-9, MW-14 and EW-1 and transmitted <br /> to a microbiologist for treatability studies. Nutrients will be added to the samples, under laboratory <br /> conditions, at various concentrations and combinations, to determine the optimal nutrient additions <br /> for the site. From past experience, laboratory studies have indicated that optimum microbial growth <br /> is maintained with combined concentrations of oxidized and reduced nitrogen (total nitrogen) at <br /> approximately 50 ppm. Present concentrations of nutrients, specifically nitrates, nitrites and <br /> ammonium, will be used as"background" or baseline samples. <br /> AGE proposes the addition of nutrients to wells MW-8,MW-9, MW-14 and EW-1, since these areas <br /> have shown the greatest concentrations of hydrocarbons (specifically benzene). We anticipate the <br /> addition of hydrogen peroxide and ammonium nitrate to each well will be in a liquid form. The <br /> nutrients will be added to each well in low concentrations, over period of 2 to 4 weeks. It is believed <br /> that the addition of high concentrations of nutrients in single injection event reduces the microbial <br /> growth at the injection point (i.e., monitoring well) and in the immediate vicinity of the well. <br /> As stated above, the specific concentrations of nutrients to be added will be based upon samples <br /> collected for treatability studies. The samples will be collected during the week of 18 December <br /> 1995; performance of the treatability studies should be completed in approximately two weeks. <br /> The addition of hydrogen peroxide to the subsurface will increase oxygen concentrations and enhance <br /> aerobic metabolism of petroleum hydrocarbons. As in soil, the addition of ammonium nitrate will <br /> provide a supplemental source of nitrogen required to achieve efficient degradational capabilities of <br /> the microorganisms. <br /> 3.4. MONITORING REQUIREMENT MODIFICATIONS <br /> Vapor monitoring has been on-going at the site since remediation was initiated in August 1994. <br /> Concentrations of oxygen,carbon dioxide and organic vapors have been monitored in most wells on- <br /> site on a weekly,twice-monthly or monthly basis. Attachment C contains graphs of oxygen, carbon <br /> dioxide and organic vapor concentrations versus time for selected wells. <br /> Although a relationship has been loosely established among CO2-02-OV concentrations in soil vapors, <br /> no clear correlation can be made between vapor concentrations and laboratory results of <br /> hydrocarbons concentrations in the soil. <br /> Monitoring for bioremediation sites should be focused upon data that will provide information on <br />