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i <br /> The third type of information used by the NRC to evaluate bioremediation is illustrated <br /> using field data collected during the pilot study Soil samples collected from P-1 and P-2 <br /> were analyzed for bacteria numbers and types All samples analyzed showed increased <br /> densities of inoculated bacteria, as compared with pre-inoculation conditions An increase <br /> in bacterial numbers indicates successful bioremediation Also measured at the site was a <br /> decrease in oxygen (a growth stimulating material) and an increase in carbon dioxide (a by- <br /> product of bioremediation) The depletion of oxygen and the production of carbon dioxide <br /> indicate that the microbes are active at the Site <br /> I <br /> Overall, the depletion of oxygen and production of carbon dioxide was greater in wells that <br /> were inoculated but not provided an air source (via low pressure blower) This can be <br /> illustrated by comparing both the field measurements and laboratory analysis of oxygen and <br /> carbon dioxide in VW-1 and VW-4 <br /> In conclusion, data collected during the 60 day"pilot study" strongly suggests that engineered <br /> bioremediation is occurring or has the potential to have occurred at the Site in response to <br /> inoculation We now recommend the full scale initiation of a bioremediation project at the <br /> Site The following section will expand on the FRP and addendum, and attempt to address <br /> remaining questions and concerns of the CVRWQCB <br /> 3.0 FULL SCALE INOCULATION <br /> 3.1 Hydrocarbon Impacted Sail <br /> GeoAudit proposes to proceed with the full site inoculation as described in the FRP and <br /> addendum However, because some wells were inoculated during the pilot study, various <br /> modifications will have to be made <br /> In the FRP, GeoAudit proposed to inject inoculum into MW-1, MW-3, MW-4 and MW-5 <br /> and VW-1 through VW-7 In an addendum to the FRP, dated October 5, 1993, GeoAudit <br /> specified that each well would receive two well volumes, a total of 432 gallons would be <br /> injected in the monitoring wells, a total of 648 gallons would be injected into the <br /> remediation (VW) wells <br /> For the full-scale inoculation, GeoAudit proposes to inject approximately 100 gallons of <br /> inoculum each into MW-1, MW-3, MW-4 and MW-5 The inoculum will be injected using <br /> packers, in a similar manner as the pilot study However, the packer will be placed only at <br /> the 30 foot depth <br /> Impacted soil in the vicinity of VW-3 and VW-7 is primarily at depths greater than 35 feet <br /> Therefore, approximately 100 gallons will be inoculated into VW-3 and VW-7 using no <br /> packer <br /> yep l Au&j s� 3 <br /> IJ31C bo hr/S91 <br />