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IA 60 day monitoring program was designed and implemented for the "pilot study " Data were collected, compiled and submitted to the CVRWQCB An overview of the momtonng program is included as Attachment A All inoculation procedures were performed under the direction of GeoAudit's consulting microbiologist, Mr James Hoeltgen It must be noted that because of restrictions in the inoculation, placed upon the project by the CVRWQCB, Mr Hoeltgen expressed at the onset severe reservations as to the value of a Iimited inoculation at the site However, in the best interest of GLDM and to initiate site remediation, the limited inoculation proceeded 2.0 PILOT STUDY Despite reservations and last minute alterations of the inoculation plan, it appears that overall, the data collected during the pilot study indicate that engineered bioremediation due to the inoculation is occurring at the Site Perhaps more importantly, the data also indicate that the bioremediation project, as designed by GeoAudrt, has the potential to remediate both impacted soil and impacted groundwater at the Site According to the National Research Council (NRC), the three types of information that show bioremediation is occurnng at a contaminated site are 1) documented loss of contaminants from the Site, 2) laboratory assays showing that microorganisms from site samples have the potential to transform the contaminants under the expected site conditions, and 3) one or more pieces of information showing that the biodegradation potential is actually realized in the Meld IThe first criterion for showing bioremediation is working at the Site is illustrated in Figures 3 and 4 Figure 2 represents a cross section, from VW-1 to VW-4, of hydrocarbon impacted ' soil prior to inoculation, Figure 3 represents the same cross section on March 28, 1994 Prior to inoculation, impacted soil was present in the area of VW-1 and VW-4 to depths in excess of 60 feet However, on March 28, 1994 (Figure 4), no impacted soil is present at depths below 28 feet near VW-1 Bonng P-1 (February 28, 1994) was drilled approximately 3 feet from VW-1, boring P-2 (March 28, 1994)was drilled approximately S feet from VW-1 The analytical data from the soil samples collected during the drilling of P-1 and P-2 indicate a reduction in the physical size of the impacted soil plume, impacted soil beneath 30 feet has been successfully remediated Bacteria were obviously present in both pilot bongs This indicates that bacteria had migrated at least S feet in 60 days The second type of information to show bioremediation is working at the site, according to the NRC, has been indicated many times using the same bacteria as was inoculated at the Site The bacteria used in the inoculum at the Site have been used in both in-situ and ex-situ situations, to successfully degrade petroleum hydrocarbons Examples of biodegradation utilizing the inoculated bacteria, under similar site conditions, were outlined in the Final Remedial Plan (FRP) prepared by GeoAudit in July of 1993 0-1b8-1 Aida gem.I.. 1�3Nea'-4nLrAs.p4 2 I