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Data collected during the quarterly soil bongs will be compiled with previous data The drilling will continue until enough data has been obtained to provide evidence for site closure The soil bonngs will be drilled to a minimum depth of 40 feet Samples will be collected at five-foot intervals from each boring All samples will be field screened using a PID. Selected samples will then be transported to a state-certified laboratory and analyzed for total petroleum hydrocarbons (TPH as gasoline) and volatile aromatics (BTE&X) in accordance with EPA methods 8015 and 8020, respectively Soil samples will also be collected and analyzed by a microbiologist for bacterial species and Inumbers No specific plate tests have been designed for the bacteria used in the inoculum Generally, bacteria must be pathogenic to have a specific test for identification, the development of bacterial identification systems is economically driven At the present time Ithere is no "economic" need to identify non-pathogens, therefore, no specific "packaged" identification systems are available IThe following identification process will be used dung the bioremediation project I1) A microbiologist will receive and perform all bacteriological assessments 2) The sample will be plated on suitable media for isolation 3) Species and strain identification will be made by using gram stain, colonial and cellular morphology, proper biochemical reactions (species dependant) and proper differential media (species dependant) The expertise of a bacteriologist will be employed I4) Bacterial numbers and different strains will be reported 5) Species present in individual samples is considered to be proprietary and confidential information is not reported It must be noted that bacterial species should not be compared to species of higher forms of life Bacteria do not sexually reproduce, bacterial species identification are based upon differential media and biochemical tests, not reproductive isolation Each bacterial species can have many different strains, these strains will differ from other strains within the same species with respect to biochemical test, reactions to differential media and metabolic activity For example, not all strains of pathogenic bacteria are pathogenic, often some can be avirulent There is much variability in bacterial species identification One example is S aureus, it is named for the golden color it exhibits However, not all S aureus are golden in color It is, Oeo1.V-1 A-61 8e.vrra I.. p i33/GM'6a-hrAt91 8