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Crof o determine BOD, oxygen the bacteria uses is calculated by comparing the amount left at the end 5 days with the known amount at the beginning. Adsorption and volatilization, followed by icrobial degradation are the primary processes for removal of soluble organics. Total Suspended Solids (TSS) is the material retained on a standard glass-fiber filter disk. Suspended solids can be categorized as volatile or fixed suspended solids. TSS can create and enhance biomat formation between the soil and effluent interface, thus restricting infiltration. The biomat, also referred to as a"clogging mat," is a biological, slimy substance which forms on the soil-effluent interface over time. This mat is composed mostly of aerobic and anaerobic bacteria and bacterial products consisting of polyuronides and polysaccharides. The biomat does have one beneficial property of removing approximately 10%of the nitrogen from gravity flow disposal systems, although this figure will not be used in the nitrate loading calculations to maintain a conservative loading concentration. Total Nitrogen is the summation of ammonia-nitrogen (NH4) and organic nitrogen (proteins, amino acids, enzymes). Since the septic tank is predominately anaerobic, nitrogen cannot exist in its oxidized state as nitrate for any length of time in the septic effluent, and if it does, it is quickly reduced to NO or NO2. If nitrate existed in the tank effluent, this would also be added to ammonium and organic nitrogen to arrive at Total Nitrogen. NLS§3.�. To quantify the anticipated nitrogen concentration for use in the nitrate loading calculations from the Produce Stand Restrooms, effluent sample test results were obtained from other comparable commercial facilities. These analytical test results (three total tests) were averaged to provide the concentrations found in Column 2, in Table 5 below. In addition, septic tank effluent concentrations from various researchers that have been published in Small and Decentralized Wastewater Management Systems, by Crites and Tchobanoglous are found in / Columns 3 and 4. These two columns illustrate the effect effluent filtration has on certain constituents of the effluent, particularly BOD and TSS. Column 5 illustrates septic tank effluent concentrations for the constituents listed that have been determined by the U.S.E.P.A. TABLE 6 Laboratory Crites and Crites and U.S.E.P.A. ANALYTE Analysis of Tchobanoglous: Tchobanoglous: Commercial Septic With Effluent Filter Without Effluent Filter System Effluent Ammonia Nitrogen(NH,-N) 103 mg/1. 40 mg/L 40 mg/L 6-18 mg/L Organic Nitrogen 10 mg/L 28 mg/L 28 mg/L 30-82 mg/L Nitrate BDL BDL BDL BDL Total Nitrogen 113 mg/L 68 mg/L 68 mg/L 35-100 mg/L Total Suspended Solids 72 mg/L 80 mg/L 30 mg/L 200-290 mg/L (TSS) Biochemical Oxygen 21 mg/l. 180 mg/L 130 mg/L 200-290 mg/L Demand(BODS) pH 7.7 N/A N/A N/A Total Alkalinity 983 mg/L 60-120 mg/L 60-120 mg/L N/A 10 C/zesney Consultiizg