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Sep-21 -9$ 05 : iup <br /> NUTRIENT ASSAYS and pH RESULTS <br /> PROTOCOL: Colorimetric assays were performed for the determinations of ammonia, <br /> nitrate and ortho-phosphate levels in water samples, per EPA manual <br /> colorimetric protocols using Hach reagents and a Gilford 340 VIS/[JV <br /> digital spectrophotometer The pH was measured with a Coming digital <br /> pH meter Mean pH values were obtained from triplicate measurements <br /> All assays conform to California CLP and Standard Water g Wastewater <br /> analytical method specifications. <br /> Ammonia Nitrate Phosphate <br /> SAMPLE SAMPLE (mg/L) (mg/L) (mg/L) <br /> GW-1 12/14/95 030 020 065 68 <br /> GW-2 12/14/95 350 3 5 700 68 <br /> BR-2 12/14/95 210 190 500 68 <br /> COMMENTS: The enumeration data indicate that hydrocarbon degraders were aresent in the <br /> groundwater prior to inoculation, but the levels were moderate The limiting <br /> factors were probably ammonia nitrogen and oxygen The inoe alation of the <br /> batch culture increased ambient levels of hydrocarbon-degrading bacteria nearly <br /> a hundred fold to over a million colony forming units per milliter Ammonia <br /> nitrogen and ortho-phosphate levels were also increased approximately 10 fold, <br /> as intended It should be noted that phosphate is readily adsorbec onto the soil <br /> micelles as well as reacting with bivalent cations in the water To provide <br /> adequate amounts of available phosphorous, it is important to amend the ground <br /> water with an excess of soluble ortho-phosphate The batch inoculation also <br /> increased the nitrate nitrogen levels slightly, but the concentration; of nitrate in <br /> the groundwater did not exceed the drinking water standard of 10 mglL <br /> Generally, proper control of ammonia nitrogen amendments to the ground water <br /> will prevent the accidental increase of nitrate levels to greater thai the 10 ppm <br /> drinking water limit The inoculation did not alter the pH of the groundwater, <br /> which remains near optimal for in situ bioremediation of petroleum <br /> Assays were performed by Sue Arve, M S , Director of Technical Services <br /> JRAL t <br /> oo9g . <br /> i '4~ Randall von Wedel, PhD <br /> Sue Arve, M S <br /> Director of Technical Services <br />