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SeR-21 -98 05 : 17P r ' yv <br /> CytoCulture <br /> ENVIRONMENTAL <br /> BIOTECHNOLOGY <br /> A DIVISION OF CffCCATVM INTERNATIONAL INC <br /> December 19, 1995 <br /> Batch Bioremediation Inoculation at Los Altos Garbage Co. Site <br /> Hydrocarbon-Degrading Bacteria Enumeration Assay Results <br /> CARBON SOURCE: Diesel (Chevron #2) and motor oil were blended in a 50 0 ratio as the <br /> sole carbon and energy sources for the growth of hydrocarbon-degrading <br /> aerobic bacteria on agar plates Gasoline (Chevron Reg ) was added to <br /> the lids to provide petroleum hydrocarbon vapors <br /> PROTOCOL: Sterile agar plates (100 x 15 mm) were prepared with minimal salts <br /> medium at pH 6 9 with 1 5% noble agar, without any other carbon <br /> sources or nutrients added A 200 µl aliquot of pasteunzed gasoline was <br /> added to absorbent paper in the plate lids to provide a vapor source of <br /> hydrocarbons Each plate was inoculated with 100 pi of each water <br /> sample, or a log dilution of each sample Triplicate plates were <br /> inoculated at sample dilutions of 100, 10-1 and 10'= for samples GW-1 <br /> and GW-2 and at sample dilutions of 10-4, 10-4 and 10-6 for sample BR-2 <br /> The plates were spread on 12/15/95 The plates were cc unted after 4 <br /> days in a humidified incubator at 30°C on 12/19/95 The plate count <br /> data are reported as colony forming units (efu) per milliliter (ml) of <br /> sample as an average of the plate count data obtained with inoculations <br /> at the 3 dilutions. <br /> SAMPLE SAMPLE COUNT HYDROCARBON-DEGrm ADERS <br /> NUMBER DATE DATE SFU PER MILLIL TER <br /> GW-1 12/14/95 12/19/95 75 x 104 <br /> GW-2 12/14/95 12/19/95 S 5 x 106 <br /> BR-2 12/14/95 12/19/95 1 2 x 108 <br /> i� <br /> 249 TEWKSBURY AVENUE POINT RICHMOND CA 94801 <br /> 510/233-0102 FAX 510/233-3777 <br />