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, ry <br /> f_ A <br /> SEQUOIA GSL f ICAL <br /> 1900 Bales Avenue • Suite LtA • Concord. Caldorma 94520 <br /> (A15)686.9600 a FAX 14151 6809689 <br /> Kaprealian Engineering,Inc. Client Project ID: Unocal-Lodi Sampled:2/28/91 <br /> P.O.Box 9% Sample Descript: Sail Camp B'i Received: 2/28/91 <br /> Benicia,CA 94510 Analysis Method: See below,Title 22 <br /> Attention: Mardo Kaprealian,P.E. Lab Number: 01020544 Reported:3/8/91 <br /> s. <br /> STATIC ACUTE HAZARDOUS WASTE BIOASSAY <br /> Staticr- ] Species: Pimephales promelas Organls-ri l•ank: 10 <br /> Cont.Flow= Common Name: Fathead Minnow Replicates: 2'r-- <br /> Mean <br /> Mean length: 30 mm Organisms/Conc.: 20 <br /> Mean weight: 0.38 g Tank Depth: 13 cm <br /> Screening El <br /> Supplier: Sticklebacks Unlimited Tank Volume: 10L <br /> Definitive Acclimation Temp.: 20 degrees C <br /> Alkalinity,m L Hardness,m L <br /> Initial Final initial Final <br /> Control 40 31 37 45 <br /> Dilution Water: Synthetic Freshwater Sok 1000 ppm 34 35 41 42 <br /> 320 ppm 38 37 43 45 <br /> 100 ppm 40 39 41 44 <br /> Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> DATE 3-2-91 3-3-91 3A-91 3-5 Ti 3-5.91 <br /> DO C pH DO C pH I#M DO C pH #M DO C pH #M DO C pH #M Total <br /> mg/L Temp Units mg/L Temp Units I Dead mg/L Temp Units Dead mg/L Temp Units Dead mg/L Temp Units Dead Dead <br /> Control 9.3 19 7.2 17.5 18 7.3 0 8.9 18 7.5 0 8.1 18 7.4 0 83.7 19 7.6 0 0 <br /> _12qff m 9.2 19 7.4 17.1 19 7.3 0 8.5 18 8.1 0 9.0 18 7.2 0 8.6 19 7.9 0 0. <br /> 180 Ppm 9.0 19 7.3 6.5 19 7.4 0 8.6 18 7.8 1 9.8 18 7.2 2 7.9 19 7.9 3 �3` <br /> 320 ppm 9.0 19 7.3 6.7 18 7.4 0 11.0 18 7.7 0 10.0 18 7.2 t 9.9 19 7.9 1 1 <br /> 560 ppm. 8.9 19 7.2 7.4 18 7.3 0 10.0 18 7,7 0 8.8 18 7.2 0 19,2 19 7.9 0 0; <br /> iU00 m 9.0 19 7.2 6.8 19 7.4 0 7.5 18 7.7 0 7.0 18 7.3 0 19.0 , 19 8.0 1 1` <br /> LC-50: >1000 LC-50 Calculation Method: Moving average angle <br /> Remarks: <br /> Analyst: R.Geckler Method Reference: Static Acute BioaEs!X Procedures for Hazardous Waste Samples, <br /> September 1987, Galitornia DeparimeFf of Fishand Game WFUL. _ <br /> SEQUOIA ANALYTICAL <br /> Arthur G.Burton @1020544.KEI <3> <br />