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SEQUOIA AALYTI AL <br /> 1900 Bates Avenue • Suds LM • Concord,California 94520 <br /> (415)666.9660 a FAX(415)686.9699 <br /> Sampled:2/28/91 <br /> Kaprealian Engineering,Inc. Client Project ID Unocal Lodi Received.2/28/91 <br /> Number; 1020547 Num <br /> P.O.Box 996 Sample Descrlpt. :Soij...Comp E <br /> Benicia,CA 94510 Analysis Method: See below,Title 22 Reported: 3/8/91 <br /> Attention: Mardo Kaprealian,P.l=. Lab , . <br /> STATIC ACUTE HAZARDOUS WASTE BIOASSAY <br /> Species: Pim e hales romelas Organlsms/Tank,: 10 <br /> Static X Reptleates:...,�-.2 a <br /> Cont.Flow[ Common Name: Fathead Minnow Organisms/Conc.: 20 <br /> Mean length: 30 mm Tank Depth: 13 cm <br /> Mean weight: 0.38 g Tank Volume: __1 L <br /> Screenings Supplier. St'scklebacks Unlimited <br /> Definitive X Acclimation Temp.: 20 degreesC <br /> Alkali)+it ,m L Hardnes�,m L <br /> Initial Final Initial Fnal <br /> Control 40 31 40 45 <br /> 1000 ppm 35 32 37 44 <br /> Dilution Water: Synthetic Freshwater Sok 320 ppm 37 31 35 47 <br /> 100 ppm 42 30 39 40 <br /> 48 Hr 72 Hr 96 Hr <br /> Initial 24 Hr 3-6-91 <br /> 3 3 91 3-4-91 3-5-91 <br /> DATE 3-2-91 <br /> DO C pH DO C pH M DO C PH #M DO C pH #M DO C pH #M Total <br /> L Tem Units Dead mg/L Temp Units head mg(L Temp Units Dead Dead <br /> mg/L Temp Units mg/L Temp Units Dead mg( P M <br /> Control 9.3 19 7.2 7.5 18 . 0 8.9 20 7.5 0 6.1 . 8.7 19 7.6 0100m 9.2 19 7.4 6.4 21 7.4 0 8.1 20 7.3 0 7.7 18 . 7.8 19 7.6 094 i9 7.3 8.2 18 7.3 .8 20 7.4 0 8.2 . 9.6 19 7.7 1320 m .2 19 7.4 7.0 21 6.8 0 8.3 20 7.5 2 . . 92 19 7.8 4 <br /> S80 m 9.2 19 7.4 7.1 20 7.4 0 8.4 20 7.6 1 7.7 18 7.4 2 8.G i9 7.7 2 :0 . <br /> ui <br /> 6.9 21 7.3 0 8.9 20 7.5 0 8.2 i8 7.6 0 8.4 19 7. <br /> 1000 m 9.0 19 7.25 0 <br /> LC-50 Calculation Method: Moving average angle <br /> LC-50: >1000 <br /> Remarks: <br /> Method Reference:Static Acute Bioassa Procedures for Hazardous Waste Samples,_ <br /> Analyst: R.Geckler a tem er 1987, a l ornia a artmenE o s an ams <br /> @1020544.KEI <5> <br />