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06 December 1995 <br /> AGE-NC Project No. 95-0100 <br /> Page 9 of 13 <br /> the costs for installation and maintenance of the system will be between $65,000 and $75,000; <br /> monthly rentaMease of the unit will likely comprise approximately $50,000 of the costs. The cost may <br /> increase substantially for the Franklyn Cole site if large volumes of supplemental fuel are required for <br /> operation of the system. <br /> 5.2. IN-SITU BIOREMEDIATION/BIOENHANCEMENT <br /> T 5.2.1. BASIC PRINCIPLES <br /> Bioremediation involves the introduction through wells of hydrocarbon-degrading microbes and/or <br /> nutrients to the hydrocarbon-impacted subsurface soil to enhance the population and activity of <br /> naturally occurring bacteria. The microbes degrade hydrocarbons and spread laterally (arid vertically) <br /> by migration and reproduction, <br /> Hydrocarbon-degrading microbes are able to metabolize hydrocarbons and assimilate the resulting <br /> organic chemicals into biomass for reproduction and growth, and yield carbon dioxide and water as <br /> waste products. Typically, the light-end hydrocarbons are degraded rapidly, and are removed from <br /> the soil first, followed by increasingly heavier hydrocarbon compounds, which are degraded at a <br /> slower rate. <br /> 5.2.2. NUTRIENT REQUIREMENTS AND TOXICITY <br /> The microbes require certain essential nutrients that are sometimes available in the subsurface. If <br /> supplemental nutrients are required, typically nitrogen and phosphorus as ammonium phosphate in <br /> liquid phase, usually only small quantities are needed. A Waste Discharge Permit is required by the <br /> Regional Water Quality Control Board for the injection of nutrients into soil and ground water. <br /> 5.2.3. INOCULATION WELLS <br /> Hydrocarbon-degrading microbes and/or nutrients are typically injected into the subsurface utilizing <br /> inoculation wells. The existing SVE wells design would facilitate thOr use for bioremediation. <br /> Installation of additional wells are not expected to be necessary. <br /> 5.2.4. MONITORING ACTIVITIES <br /> Following the inoculation event, the inoculation wells should be monitored periodically for several <br /> months for field measurements of organic vapors, carbon dioxide, and oxygen, Quarterly or semi- <br />