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East Bay MUD - 3 - 10 March 1995 <br /> Memo #2 <br /> I. The "zero line" has been estimated by installation of monitoring wells, however, the distance <br /> between contaminated wells and uncontaminated wells is a minimum of 50 feet and a <br /> maximum of 120 feet. Fine-tuning a remediation system based on the known "zero line" may <br /> be difficult. <br /> - Is fine-hmirg of the rer.iec iadon system necessary? <br /> - How nuich of the contansiriation plume is going to be irnpbctctw.d by the <br /> mystern? <br /> ffiORIIVIED�ATION PROCEDURE <br /> A. According to the RAP, three enzymes will be used to assist in the treatment of the <br /> hydrocarbon contamination. The enzymes, monomethanoxigenase, alcoholdehydrogenase, and <br /> aldehydehydrogenase, have been isolated from bacteria that produced them. Enzymes are <br /> highly specific for the reactions they catalyze. These enzymes assist in the break down of <br /> methane, alcohol, and aldehydes. <br /> - How are the three added enzymes able to assist in the break dawn of petroletmt <br /> hydrocaaborns? <br /> - Hary are the enzymes mile to break down hydrncczrbans outs7ide the bacterid cells m*hout <br /> at energy source? <br /> - Do the three enzymes listed require cofactors to react with.hydrocaabons? <br /> - If the enzymes do react with hydroccabons, u*hout additional energy to complete <br /> hy+drocabon bmakdou r; wiz! the enzymes be inactivated m the reactions? <br /> - It a7peow that the enzymes are acting more as a sudhctart than a catalyst Whit evidence <br /> is provided to the contrary? <br /> B. The RAP states that the enzymes were used for remediation of creosote and dioxin. <br /> - FiTkwt haw the three listed enzymes would dist m the break down of creosote and <br /> diax&L <br /> - How do enzymes that breads down creosote and a mxxr; break down petroleum <br /> hydrocabons as uvU? <br /> C. According to the RAP, the enzymes are "stabilized and concentrated into crystalline form". <br /> During application at the site, the enzymes are "reconstituted" with ground water. <br /> - Fxplain these procedums. <br /> D. Biosurfactants, amino acids, and air are added, mixed with the enzymes and extracted ground <br /> water, and reinjected into the subsurface. <br /> E. According to the RAP, biosurfactants enhance the contact time between the enzymes and the <br /> contaminants. <br /> . FVk*t how this procedum workL <br /> - What is the stofi bmt? Is it biodegradable? <br /> - Will the siafoctarrt act as a soil washer, transfenirg hydrocarbons from soil to ground <br /> water? <br /> WIU the action of the naryactart pmnw t detection of hydrocabors by the Ir maMW <br /> F. According to the RAP, amino acids will promote the rapid growth of the natural bacterial <br /> population. <br /> - Is ernof the na tad bacterid population a nrt1por objective of this project? <br /> - Will the same bacteria drat use amino acids as a food source, use BTEX sound wog s as a <br /> food source? <br />