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i with Teflon-lined lids and placed on ice in a cooler until shipment, with chain of custody document, to McCampbell Analytical, Inc in Pacheco, California The soil sample analytical results showed TPH(g) concentrations of 5,900 mg/kg (20') and 1,100 mg/kg (35'), benzene concentrations of<0 5 mg/kg (20') and 0 53 mg/kg (35'), MTBE concentrations of<10 mg/kg (20') and <5 0 mg/kg (35'), and total BTEX concentrations of 527 mg/kg (20') and 194 63 mg/kg (35') A copy of the analytical report is included in Appendix D I Soil samples were collected from well boring IAS-1 for average permeability, organic content, and specific gravity analysis Samples were retained for analysis at depths of 25 and 40 feet bgs The 25 foot sample is representative of the vadose zone soils and the 40 foot sample is representative of the saturated zone soils The samples were sealed in glass bars and placed on ice in a cooler until shipment, with chain of custody document, to Cooper Testing Laboratory in Mountain View, California IThe 25 foot soil sample showed an average permeability of 2 E-08 centimeters per second (cm/sec), an organic content of 1 8 percent (%), and a specific gravity of 2 75 The 40 foot soil sample showed an average permeability of 9 E-06 cm/sec, an organic content of 18 %, and a specific gravity of 2 73 A copy of the analytical report is included in Appendix D Enhanced Bioremediation Pilot Test Soil samples for the treatability studies were collected from the source area (well boring VE-1) and from an unaffected area of the site (boring SB-14) Two samples were retained from well boring VE-1 at depths of 25 and 40 feet bgs These depths correlated to the highest field photoionization detector(PID)readings in VE-1 Two samples were retained from boring SB-14 Eat depths of 25 and 40 feet bgs No field PID readings were detected at these depths The samples were sealed in brass tubes and placed in a cooler for shipment, with chain of custody document, to BioJet Innovative Remediation Technologies in Ione, California A Copy of the Enhanced Bioremediation pilot test report is included in Appendix E ' Physicochemical and microbiological analyses were conducted on each sample for nutrient tracking of nitrate, ammonium, ortho-phosphate, and potassium concentrations, pH, and microbial evaluations The analytical results revealed that the samples collected within the I impacted and unimpacted areas yielded very low recoveries of both heterotrophic and selective microorganisms, indicating inadequate levels of naturally occurring nutrients for acceptable bioremediation to occur Bench studies were run on the two samples collected from the source area (VE-1) to test for the effects of oxygen and nutrients on the stimulation of selective microbes The tests took place over a 21-day period and involved adding various levels of nutrients or oxygen to the samples Microbial growth was then tested at 4, 7, 14, and 21 days The bench test results indicated that both the nutrient and oxygen augmentation appear to enhance activity for the microbial population Increases in selective microbial counts (microorganisms capable of specifically degrading the target contaminant) were observed with both oxygen (76 fold increase) and nutrient (320 fold increase) augmentation RFGUI� O 10 V ILICES~Z