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Pacific EcoRisk Environmental Consulting and Testing <br />1. INTRODUCTION <br />GeoAnalytical Laboratories, Inc. (GeoAnalytical) has contracted Pacific EcoRisk (PER) to <br />conduct a screening -level Title 22 Hazardous Waste acute aquatic toxicity evaluation of a waste <br />sample designated "Waste Water Pond 1,2 Composite" that was delivered to the PER testing <br />laboratory on December 20, 2018. This evaluation consisted of performing a 96 -hour toxicity <br />test with juvenile fathead minnows (Pimephales promelas). This report describes the <br />performance and results of this test. <br />2. ACUTE TOXICITY TEST PROCEDURES <br />This testing followed the guidelines established by the California Dept. of Fish & Game manual <br />"Static Acute Bioassay Procedures for Hazardous Waste Samples" (Polisini and Miller 1988), <br />with additional guidelines as provided by the California Dept. of Health Services (Janna White, <br />personal communication). <br />2.1 Sample Receipt and Handling <br />The waste sample designated "Waste Water Pond 1,2 Composite" was delivered to the PER <br />testing laboratory on December 20, 2018, where it was placed under refrigeration at 4°C until <br />being used to prepare the test solutions. The chain -of -custody record for the collection and <br />delivery of this waste sample is provided in Appendix A. <br />2.2 Preparation of the Test Media <br />The Lab Water Control medium for this test consisted of synthetic US EPA soft water, prepared <br />by adding reagent grade salts to Type 1 lab water (reverse -osmosis de -ionized water). The waste <br />sample was homogenized, after which sub -samples of waste were mixed with the Lab Water <br />Control medium for six hours, using a rotary mixer. After mixing, these preliminary solutions <br />were used to prepare test solutions at sample concentrations of 250, 500, and 750 mg/L. <br />2.3 Acute Toxicity Testing with Fathead Minnows <br />The fathead minnows used in this test were obtained from a commercial supplier (Thomas Fish <br />Company, Anderson, CA). These fish were maintained in aerated aquaria containing EPA <br />synthetic soft water at 20°C prior to their use in this test. <br />This hazardous waste test was run at sample concentrations of 250, 500, and 750 mg/L, with a <br />Lab Water Control treatment consisting of the same synthetic EPA soft water. There were two <br />replicates at each test treatment, each replicate consisting of 9.2 L of media in a 10-L container. <br />Water quality characteristics (pH and dissolved oxygen [D.O.]) were determined for the test <br />solution in each test replicate prior to initiation of this test. <br />Page 1 �))� <br />4/13 (( <br />