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Pacific EcoRisk <br />Environmental Consulting and Testing <br />The test was initiated with the random allocation of 10 juvenile fathead minnows into each <br />replicate container. These containers were placed in a temperature -controlled room at 20°C, <br />under fluorescent lighting on a 16L:8D photoperiod. <br />Each day, each replicate container was examined, and the number of live fish in each was <br />recorded. Routine water quality characteristics (temperature, pH, and D.O.) of the treatment <br />waters were measured and recorded for each replicate container each day. <br />After 96 (±2) hours, the test was terminated and the number of live fish in each replicate <br />container was determined. The fish in a randomly selected Lab Control treatment replicate were <br />then euthanized and measured for total length and weighed. The resulting survival data for the <br />waste treatments were evaluated to determine the LC50 using CETISTI (TidePool Scientific <br />Software, McKinleyville, CA). <br />3. RESULTS <br />The results of this test are summarized in Table 1. The LC50 of this sample was >750 mg/L, <br />which is greater than the hazardous waste classification threshold of 500 mg/L, as defined by CA <br />Title 22 CCR §66261.24. Accordingly, the sample should not be considered a hazardous waste <br />with respect to acute aquatic toxicity. The test data and summary of statistics for this test are <br />presented in Appendix B. <br />Table 1. Results of the hazardous waste toxicity evaluation of the waste sample "Waste Water <br />Pond 1, 2 Composite". <br />Waste Sample Treatment Level <br />Mean % Survival <br />Lab Control <br />100 <br />250 mg/L <br />100 <br />500 mg/L <br />100 <br />750 m /L <br />-'summary of Key Statistic <br />100 <br />LC50 = <br />1 <br />>750 mg/L* <br />* Due to the absence of significant mortalities, the LC50 cannot be calculated, but can be determined by inspection <br />to be >750 mg/L. <br />Page 2 �))� <br />5/13 (( <br />