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J31VIlUZUIUUUN VVdbVZ 1VldUdgVJ11U111 raga O V1 7 always necessary. iv. Always remember once the wrapping is compromised sterility is lost. v. Autoclave and daily maintenance: • Gauges, temperature recorders, etc. must always be calibrated periodically to assure accuracy. • Wash chamber periodically with trisodium phosphate or a mild detergent. Never use strong abrasive or steel wool. • Rinse chamber with tap water and dry with lint free cloth. • Remove and clean drain strainer. • Wash, rinse, and dry loading carts as indicated. • Flush and clean periodically chamber discharge. • Cracks in minor ones should be repaired, if possible. f. Many biohazardous wastes that have multiple hazards must not be steam sterilized because of the potential for exposure of equipment operators to toxic, radioactive, or other hazardous chemicals. Infectious wastes that must not be steam sterilized includes those that contain anti -neoplastic drugs, toxic, chemicals, radioactive, or chemicals that would be volatilized by steam. Mixed wastes containing tritium, carbon-14 and other radionuclides can be autoclaved safety. Waste containing flammable or reactive chemicals must not be autoclaved. Care must be taken to avoid contamination of the equipment. Autoclaves used for processing radioactive waste must be labeled with the radiation symbol. g. Persons involved is steam sterilizing infectious wastes must be educated in proper techniques to minimize personal exposure to hazards posed by these wastes. These techniques include use of protective equipment, minimization of aerosol formation, prevention of spillage of waste during autoclave formation, prevention of spillage of waste during autoclave loading and unloading, emergency response and vapor inhalation. h. Suggested Temperatures, Pressures and Times: o Laundry: 250°F(121 °C) for 30 minutes with 15 minutes pre -vacuum of 27 inches of Hg. o Trash: 250°F(121 °C) for 1 hour with 15 minutes re -vacuum of 25 inches of Hg. o Glass: 250°F(121 °C) for 1 hour with 15 minutes pre -vacuum of 27 inches of Hg. o Liquids: 250°F(121 °C) for 1 hour for each gallon. o Tissue: 250°F(121 °C) for 1 hour for each pound with 15 minutes of pre - vacuum of 27 inches of Hg. o Bedding: 250°F(121 °C) for 8 hours with 15 minutes pre -vacuum of 27 inches of Hg. MiTir. . . . , 1. All autoclaves, steam sterilizers, retorts, and pressure vessels operating on the university must be routinely inspected, certified and serviced. Note: Certification for boilers is done by the State Department of Labor and Industrial Relations, Division of Occupational Health and Safety, Boiler and Elevator Inspection Bureau). file://CADocuments and Settings\OwnerWy DocumentsT&PUnfection Control 2005\Bioha... 5/5/2005