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tip. The net is regrasped with the free hand below the clump of captured flies. The entire net <br /> tip with flies is inserted into a killing jar and the lid put on loosely (so as not to tear the net <br /> fabric) until the flies are immobilized., The specimens are emptied from the net into another <br /> killing jar and kept there until dead. While the first catch is being killed, another sample can <br /> be taken. The dead flies are put into a labeled box with soft tissue for transport to the <br /> laboratory. Flies are collected until the inspector believes that a representative sample has been <br /> acquired. <br /> Other methods are mentioned in performance standards for capturing adult flies. <br /> Experimentation with other methods including bait traps and sticky-fly tapes is being conducted <br /> this season, and specific techniques and procedures will be provided at a later time. <br /> The first identification step should be to determine if the flies are members of the "domestic" <br /> families (Muscidae, Anthomyiidae, C-alhohoroidae, Sarcophagidae, and Drosophilidae). <br /> Domestic flies should then be identified at least to genus znd to species where possible. A list <br /> of suggested identification keys for domestic dies is arl_-.zhed. Staff of the appropriate district <br /> office to the Vector Biology and Controi SE--ton -ie available to keep in the species <br /> identification when necessary. <br /> CrawlerFlies <br /> Crawler flies are newly emerged adult .'—,-s which have not expanded their wings and which run <br /> about rapidly when disturbed. The performance standards call for routine "observations" for <br /> crawler flies but the extent of the observation is left up to the inspector. A minimum <br /> interpretation of this requirement would be that the inspector know what crawler flies look like <br /> and be alert to their presence when conducting other inspection activities at the site. In addition, <br /> the inspector should determine where fly pupae might occur on a specific site, and view these <br /> areas for possible emerging flies at each visit. For example, at a site with an advancing working <br /> face, the inspector should observe arms near the lateral edges of where the working face was <br /> located dh= to eight days prior to the present in tion. The rationale in this case is that <br /> mature larval stages of the flies may have migrated from the uncovered wastes at night to pupate <br /> in soil at the lateral edges of the open face. (Flies pupating in front of the open face would be <br /> buried by the incoming wastes the next day.) <br /> Tf crawler flies are observed, their numbers neer square yard should be counted. Tle fly grill <br /> Is a square yard in area, ana can oe user co estimate the required dimensions. in aadition, <br /> specimens of the crawler flies should be collected for species identification. <br /> 'The recommended killing agent is ethyl acetate. Insects should not be exposed to this <br /> chemical any longer than is necessary to kill them, otherwise they will be discolored and difficult <br /> to identify. <br /> M:WM,217-ZALT=V B-4 <br />